Streptococcus mutans is a common principal causative agent of dental caries. In this communication, we describe that the antibodies raised against purified dextransucrase effectively inhibited the growth of S. mutans. The purified enzyme showed 58-fold enrichment, 17.5% yield and a specific activity of 3.96 units/mg protein. Purified IgG fraction of the antibody showed significant affinity with the antigenic protein. Immunotritation of the enzyme with dextransucrase antibody showed a gradual increase in inhibition of dextransucrase activity. The growth of S. mutans was also inhibited by 85% in the presence of 28 μg of IgG fraction of the antibody. Antibodies also impaired glucosyltransferase activity (72.8%) and biofilm formation by 92.6% in S. mutans. Western blot analysis revealed no cross reactivity with the various tissues of mice, rat, rabbit and humans. Dot blot analysis showed little reactivity with Lactobacillus acidophilus and Staphylococcus aureus and there was no reactivity with other bacterial strains like Enterococcus faecalis, Escherichia coli and Salmonella typhimurium. These findings suggest that antibody raised against dextransucrase exhibit inhibitory effects on the growth of S. mutans and biofilm formation with no reactivity with various mammalian tissues, thus it could be an effective anticariogenic agent.

中文翻译：

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针对葡聚糖转移酶产生的抗体在变形链球菌中具有潜在的抗静电特性。

变形链球菌是龋齿的常见主要病原体。在这种交流中，我们描述了针对纯化的葡聚糖转氨酶产生的抗体有效抑制变形链球菌的生长。纯化的酶显示出58倍的富集，17.5％的收率和3.96单位/毫克蛋白质的比活。抗体的纯化的IgG部分显示出与抗原蛋白的显着亲和力。右旋糖核酸酶抗体对酶的免疫作用显示出右旋糖核酸酶活性的抑制作用逐渐增加。在存在28μg抗体IgG部分的情况下，变形链球菌的生长也被抑制了85％。在变形链球菌中，抗体也使葡糖基转移酶活性（72.8％）和生物膜形成减少了92.6％。蛋白质印迹分析显示与小鼠，大鼠，兔子和人类。斑点印迹分析显示与嗜酸乳杆菌和金黄色葡萄球菌几乎没有反应，与其他细菌菌株如粪肠球菌，大肠杆菌和鼠伤寒沙门氏菌也没有反应。这些发现表明，针对葡聚糖转移酶产生的抗体对变形链球菌的生长和生物膜形成具有抑制作用，并且与多种哺乳动物组织没有反应性，因此它可能是有效的抗龋剂。