Phaeomoniella chlamydospora and wood‐rotting basidiomycete fungi, namely Fomitiporia spp., are known esca pathogens. However, the effect of their mixed infections and the sequence of infection on disease development is unclear. To determine the effects of single and co‐inoculations on symptoms, potted Vitis vinifera ‘Crimson Seedless’ was inoculated with P. chlamydospora either alone or in combination with one of four basidiomycetes: Coprinellus radians, Fomitiporia langloisii, F. polymorpha and the novel species Tropicoporus texanus. Basidiomycetes were isolated from vines with foliar symptoms of esca in California and Texas. In sequential co‐inoculations, the effects of different sequences of infection (P. chlamydospora first, basidiomycete 6 months later; and vice versa) were tested, compared to simultaneous co‐inoculations. Plants inoculated with P. chlamydospora either alone or in combination with a basidiomycete (in any sequence) did not differ significantly in the length of black‐streaking lesions. In plants inoculated only with a basidiomycete, the appearance of large brown lesions, coupled with the absence of this wood symptom from control plants, suggests that C. radians, F. langloisii and T. texanus are pathogenic. Foliar symptoms resembling those of esca in the field (marginal and/or interveinal scorching, combined with red and/or yellow discoloration) were statistically more frequent among plants inoculated with F. polymorpha or T. texanus, either simultaneously or following P. chlamydospora, compared to single inoculations. Sequential co‐inoculations of a basidiomycete before or after P. chlamydospora were associated with similar lesion lengths, suggesting that basidiomycetes may not require infection by P. chlamydospora in order to extensively colonize the wood.

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担子菌真菌在葡萄干病中的作用

沙眼衣原体和腐烂的担子菌真菌，即Fmitiporia spp。，是已知的埃斯卡病原体。但是，它们的混合感染和感染顺序对疾病发展的影响尚不清楚。为了确定单次接种和联合接种对症状的影响，盆栽葡萄“ Crimson Seedless”单独或与四种担子菌中的一种一起与衣原体一起接种：鸡腿菇，弧形拟南芥，Fmitiporia langloisii，F。 多形和新物种Tropicoporus texanus。在加利福尼亚州和德克萨斯州，从带有叶面症状的葡萄藤中分离了担子菌。在顺序联合接种中，与同时联合接种相比，测试了不同感染顺序（首先是衣原体假单胞菌，6个月后是担子菌，反之亦然）的影响。单独或联合担子菌（以任何顺序）接种衣原体假单胞菌的植物在黑色斑纹的长度上没有显着差异。仅与担子菌接种的植物，棕色大损伤的外观，加之没有从对照植物这种木材症状的，表明C.弧度，F. langloisii和T. texanus是致病的。从统计学上讲，在同时接种F. polymorpha或T. texanus的植株中，同时或在接种P. chlamydospora或F. chlamydospora后，在田间出现类似于esca的叶片症状（边缘和/或间壁灼热，并伴有红色和/或黄色变色）。与单次接种相比。在衣原体假单胞菌之前或之后对担子菌的连续共接种与相似的病灶长度有关，这表明为了广泛地定居木材，担子菌可能不需要被衣原体感染。