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Bidirectional colonization and biofilm formation by Erwinia psidii in eucalypt plants
Plant Pathology ( IF 2.3 ) Pub Date : 2020-02-05 , DOI: 10.1111/ppa.13136
N. P. Caires 1 , L. M. S. Guimarães 1 , P. S. Hermenegildo 2 , F. A. Rodrigues 3 , J. L. Badel 4 , A. C. Alfenas 1
Affiliation  

Dieback and wilt caused by Erwinia psidii is an emerging disease that has been causing considerable damage in eucalypt plantations. Because it is a recently emerged disease, several aspects of the bacterial interaction with its host still remain to be elucidated. In this work, we studied the E. psidii colonization and biofilm formation in eucalypt tissues by specific detection using PCR and scanning electron microscopy (SEM). The results indicate that the bacterium is able to translocate in stem tissue mainly acropetally, although movement in the basipetal direction was also observed to a lesser extent, always through the xylem. No colonization of phloem tissues was observed. In addition to colonizing the xylem, E. psidii colonized the parenchymatous tissue. The bacterium formed cell aggregates enveloped by fibrillar material that evolved into complex, well‐structured biofilms in stem and leaf tissues. In contrast, no biofilm formation was observed on abiotic surfaces. These observations suggest that biofilm formation plays an important role in the elicitation of dieback and wilt symptoms caused by E. psidii on eucalypt plants. This study not only shows ultrastructural aspects of the E. psidii communities but also tissue damage in eucalypt plants that was associated with the presence of bacterial aggregates and formation of tyloses.

中文翻译:

Erwinia psidii 在桉树植物中的双向定殖和生物膜形成

由 Erwinia psidii 引起的枯萎和枯萎病是一种新出现的疾病,已经在桉树种植园中造成了相当大的破坏。因为它是一种最近出现的疾病,细菌与其宿主相互作用的几个方面仍有待阐明。在这项工作中,我们通过使用 PCR 和扫描电子显微镜 (SEM) 的特异性检测研究了桉树组织中的 E. psidii 定植和生物膜形成。结果表明,该细菌能够在茎组织中主要向顶叶移位,尽管也观察到较少程度的向基叶方向的运动,但总是通过木质部。没有观察到韧皮部组织的定植。除了定植木质部外,E. psidii 还定植了薄壁组织。这种细菌形成了被纤维状物质包裹的细胞聚集体,这些物质在茎叶组织中进化成复杂的、结构良好的生物膜。相反,在非生物表面上没有观察到生物膜形成。这些观察结果表明,生物膜形成在桉树植物上由 E. psidii 引起的枯萎和枯萎症状的引发中起着重要作用。这项研究不仅显示了 E. psidii 群落的超微结构方面,而且还显示了桉树植物的组织损伤,这与细菌聚集体的存在和 tyloses 的形成有关。桉树植物上的 psidii。这项研究不仅显示了 E. psidii 群落的超微结构方面,而且还显示了桉树植物的组织损伤,这与细菌聚集体的存在和 tyloses 的形成有关。桉树植物上的 psidii。这项研究不仅显示了 E. psidii 群落的超微结构方面,而且还显示了桉树植物的组织损伤,这与细菌聚集体的存在和 tyloses 的形成有关。
更新日期:2020-02-05
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