Chickpea chlorotic dwarf virus (CpCDV; genus Mastrevirus, family Geminiviridae) is one of the most important legume‐infecting viruses with a wide host range and geographic distribution in Africa and Asia. In Iran, CpCDV is common in chickpea (Cicer arietinum), but there is limited information about diversity and infections in plants of other legume species. In the current study, a total of 1671 leaf samples from different pulse crops with symptoms were collected in nine provinces of Iran, and the CpCDV infection status was tested by PCR and/or rolling circle amplification (RCA), resulting in the detection of CpCDV in samples of chickpea, lentil (Lens culinaris) and faba bean (Vicia faba) from different regions. Sequence analysis of complete genomes of 18 isolates recovered by digestion of RCA products revealed infection with isolates of the strains CpCDV‐A and CpCDV‐F in chickpea, lentil and faba bean. Phylogenetic analysis showed that the Iranian isolates of CpCDV were closely related to previously sequenced isolates of CpCDV‐A and CpCDV‐F. To the authors' knowledge, this is the first report of CpCDV‐F in Iran. Using agroinoculation with infectious clones for one isolate each of CpCDV‐A and CpCDV‐F, infectivity was confirmed in both faba bean and chickpea, with plants developing leaf curling and/or yellowing. Both infectious clones also successfully infected Nicotiana benthamiana resulting in mild yellowing and intensive leaf curling for CpCDV‐A, and dark‐green mosaic, dwarfing and mild leaf curling for CpCDV‐F.

中文翻译：

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伊朗豆类植物鹰嘴豆绿矮化病毒的多样性和发生

鹰嘴豆褪绿矮缩病毒（CpCDV;属马斯特，家庭联体病毒科）是在非洲和亚洲广泛的宿主范围和地理分布的最重要的豆类的感染病毒之一。在伊朗，CpCDV在鹰嘴豆（Cicer arietinum）中很常见，但是关于其他豆科植物的多样性和感染的信息却很少。在本研究中，从伊朗的九个省份收集了不同症状的不同豆类作物的1671个叶样品，并通过PCR和/或滚环扩增（RCA）检测了CpCDV感染状况，从而检测到了CpCDV鹰嘴豆，小扁豆（Lens culinaris）和蚕豆（野豌豆）的样品中）。通过RCA产物消化回收的18个分离株的完整基因组的序列分析显示，鹰嘴豆，小扁豆和蚕豆中分别感染了CpCDV-A和CpCDV-F菌株。系统发育分析表明，伊朗的CpCDV分离株与先前测序的CpCDV-A和CpCDV-F分离株密切相关。据作者所知，这是CpCDV-F在伊朗的首次报道。使用感染性克隆的农杆菌接种分别分离CpCDV-A和CpCDV-F的一种分离株，在蚕豆和鹰嘴豆中均确认到了感染性，并且植株叶片卷曲和/或发黄。这两个传染性克隆也成功感染了本氏烟草 导致CpCDV‐A出现轻微的泛黄和浓密的叶子卷曲，以及CpCDV‐F出现深绿色的镶嵌，矮化和轻微的卷曲。