Efforts to control viral diseases of grapevine include the production of certified material and development of virus‐resistant transgenic grapevines. However, effective antiviral agents, once the viruses have infected the plants, are still lacking. This study shows that a crude garlic extract has significant antiviral activity against grapevine viruses. Replication of grapevine leafroll‐associated virus 2 (GLRaV‐2) was obviously inhibited in grapevine cv. Cabernet Sauvignon calli treated with diluted (1:100) garlic extract. The relative RNA levels of GLRaV‐2 and grapevine fleck virus (GFkV) in cv. Summer Black grapevine in in vitro‐grown plantlets 10 days after treatment with diluted (1:100) garlic extract were about 22% and 20%, respectively, of that in controls. The viral RNA accumulation of GLRaV‐2, GFkV, grapevine virus A (GVA), grapevine fanleaf virus (GFLV) and grapevine rupestris stem pitting‐associated virus (GRSPaV) in field‐grown grapevine cv. Centennial Seedless plants sprayed with diluted (1:100) garlic extract were about 31–40%, 26–38%, 18–31%, 17–42% and 15–18%, respectively, of that in controls. Moreover, the garlic extract treatment led to a significant decrease in viral RNA accumulation of GLRaV‐3, GLRaV‐2, GVA, GFkV, GFLV, GRSPaV and grapevine Pinot Gris virus in pot‐grown grapevine cv. Shine Muscat plants, and viral disease symptoms in these plants were obviously attenuated. In addition, this extract significantly induced expression of pathogenesis‐related protein genes and stimulated activity of antioxidant enzymes in grapevines. Taken together, these results indicate that the crude garlic extract acts as a significant inhibitor against a broad range of grapevine viruses.

中文翻译：

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大蒜粗提取物显着抑制葡萄病毒的复制

控制葡萄病毒性疾病的工作包括生产认证材料和开发抗病毒的转基因葡萄。但是，一旦病毒感染了植物，仍然缺乏有效的抗病毒剂。这项研究表明，大蒜粗提取物对葡萄病毒具有显着的抗病毒活性。葡萄叶片相关病毒2（GLRaV-2）的复制在葡萄树中明显受到抑制。赤霞珠愈伤组织用稀释的（1：100）大蒜提取物处理。牛体内GLRaV-2和葡萄斑点病毒（GFkV）的相对RNA水平。夏季黑葡萄体外用稀释的（1：100）大蒜提取物处理后10天长出的幼苗分别约为对照的22％和20％。GLRaV-2，GFkV，葡萄病毒A（GVA），葡萄扇叶病毒（GFLV）和葡萄墨杆茎麻点相关病毒（GRSPaV）在田间生长的葡萄简历中的病毒RNA积累。喷洒稀释（1：100）大蒜提取物的百年无核植物分别约为对照的31–40％，26–38％，18–31％，17–42％和15–18％。此外，大蒜提取物处理导致盆栽葡萄cv中GLRaV-3，GLRaV-2，GVA，GFkV，GFLV，GRSPaV和葡萄黑皮诺格里斯病毒的病毒RNA积累显着降低。使马斯喀特植物发光，这些植物中的病毒病症状明显减弱。此外，该提取物显着诱导了发病机理相关蛋白基因的表达，并增强了葡萄中抗氧化酶的活性。综上所述，这些结果表明，粗大蒜提取物可作为多种葡萄病毒的重要抑制剂。