Abstract Spot blotch caused by Bipolaris sorokiniana is an important disease in barley worldwide, causing considerable yield losses and reduced grain quality. In order to identify QTL conferring resistance to spot blotch, a highly diverse worldwide barley set comprising 449 accessions was phenotyped for seedling resistance with three isolates (No 31, SH 15 and SB 61) and for adult plant resistance at two locations (Russia and Australia) in two years. Genotyping with the 50 k iSelect barley SNP genotyping chip yielded 33,818 informative markers. Genome-wide association studies (GWAS) using a compressed mixed linear model, including population structure and kinship, revealed 38 significant marker-trait associations (MTA) for spot blotch resistance. The MTA corresponded to two major QTL on chromosomes 1H and 7H and a putative new minor QTL on chromosome 7H explaining between 2.79% and 13.67% of the phenotypic variance. A total of 10 and 14 high-confidence genes were identified in the respective major QTL regions, seven of which have a predicted involvement in pathogen recognition or defence.

中文翻译：

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大麦 (Hordeum vulgare) 多样性集中的全基因组关联研究揭示了对斑点斑点病 (Bipolaris sorokiniana) 抗性的基因座数量有限

摘要 Bipolaris sorokiniana 引起的斑点斑病是世界范围内大麦的重要病害，造成相当大的产量损失和谷物质量下降。为了鉴定赋予斑点斑点病抗性的 QTL，对包含 449 个种质的高度多样化的全球大麦组进行了表型分析，用于三个分离株（No 31、SH 15 和 SB 61）的幼苗抗性和两个位置（俄罗斯和澳大利亚）的成株抗性) 两年内。使用 50 k iSelect 大麦 SNP 基因分型芯片进行基因分型产生了 33,818 个信息标记。使用压缩混合线性模型（包括种群结构和亲缘关系）的全基因组关联研究 (GWAS) 揭示了 38 个显着的斑斑抗性标记性状关联 (MTA)。MTA 对应于染色体 1H 和 7H 上的两个主要 QTL 和染色体 7H 上推定的新次要 QTL，解释了 2.79% 至 13.67% 的表型变异。在各自的主要 QTL 区域共鉴定出 10 个和 14 个高置信度基因，其中 7 个预测参与病原体识别或防御。