Flooding stress causes a significant yield reduction in soybean. The early growth of soybean in Korea coincides with the rainy season, potentially exposing to flooding stress. Greenhouse experiments were conducted to map the quantitative trait loci (QTL) for flooding tolerance in soybean and to identify and investigate candidate genes near the QTL hot spots. Flood stress was imposed at V1–V2 stage on a recombinant inbred line population (‘Paldalkong’ × ‘NTS1116’), and leaf chlorophyll content (CC) and shoot dry weight (DW) were measured under control and flooded conditions. The genetic map was constructed using 180K Axiom^® SoyaSNP markers. The QTL were analysed under control and flooded conditions as well as for index (ratio of CC or DW under flooded to control, CCI and DWI) and flooding tolerance index (FTI, mean of CCI and DWI). A total of 20 QTL with LOD scores 3.59–19.73 causing 5.8%–33.3% phenotypic variation were identified on nine chromosomes. Chromosomes 10, 12 and 13 harboured relatively more stable QTL. Results of this study could be useful to further understand the genetic basis of soybean's flooding tolerance and applied in breeding programmes.

中文翻译：

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大豆重组自交系群体早期耐旱性的数量性状位点定位。

洪水压力导致大豆单产大幅下降。韩国大豆的早期生长与雨季相吻合，可能会遭受洪水压力。进行了温室实验，以绘制定量特征位点（QTL）来分析大豆的耐水性，并鉴定和研究QTL热点附近的候选基因。在重组自交系种群（“ Paldalkong”ד NTS1116”）上，在V1-V2阶段施加了洪水胁迫，并在控制和淹水条件下测量了叶绿素含量（CC）和枝干重量（DW）。使用180K公理构建遗传图谱^®SoyaSNP标记。分析了在控制和淹没条件下的QTL，以及指数（淹没到控制之下CC或DW的比率，CCI和DWI）和淹没耐受指数（FTI，CCI和DWI的平均值）。在9条染色体上鉴定出总共20个QLO，LOD得分为3.59–19.73，导致表型变异为5.8％–33.3％。染色体10、12和13具有相对更稳定的QTL。这项研究的结果可能有助于进一步了解大豆的耐水遗传基础，并在育种计划中得到应用。