In this study, we have generated more than 12,000 activation‐tagged mutants in a high‐yielding indica rice variety, 'BPT 5204', employing maize Ac/Ds system. Different transgenic plants obtained were analysed based on expression patterns of green fluorescence protein (GFP), red fluorescence protein (RFP), herbicide (Basta) tolerance and molecular analyses. T₁ seeds of pSQ5 and pSQ5‐bar transgenics, when germinated separately on hygromycin (50 mg/L) and phosphinothricin (5 mg/L) containing medium, revealed a segregation of 3 tolerant : 1 susceptible plants. The germinal transposition frequency of Ds element in different T₂ progeny of rice plants was found to be about 18.0%. Different stable tagged mutants exhibited marked increases in plant height, number of tillers, leaf size, panicle size, seed size and number of grains per plant. The overall results indicate that the genes associated with these traits are upregulated by the enhancer element in activation‐tagged mutants. As such, the various tagged mutant lines appear promising and serve as a valuable genetic resource for identification of key genes determining different agronomic traits of rice.

中文翻译：

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优质in稻品种中大量带有激活标签的突变体的开发

在这项研究中，我们已经在高产产生超过12000激活标记突变体籼稻水稻品种“BPT 5204”，采用玉米AC / Ds的系统。基于绿色荧光蛋白（GFP），红色荧光蛋白（RFP），除草剂（Basta）耐受性和分子分析的表达模式分析了获得的不同转基因植物。当pSQ5和pSQ5- bar转基因的T ₁种子分别在含有潮霉素（50 mg / L）和草丁膦（5 mg / L）的培养基上发芽时，显示出3种耐性植物的分离：1种易感植物。不同T ₂中Ds元素的生发转座频率发现水稻植物的后代约为18.0％。不同的稳定标记的突变体在植物高度，分till数，叶大小，穗大小，种子大小和每棵植物的谷粒数量上显示出明显的增加。总体结果表明，与这些性状相关的基因被激活标记突变体中的增强子元件上调。这样，各种标记的突变体系看起来很有希望，并作为鉴定决定水稻不同农艺性状的关键基因的宝贵遗传资源。