Faba bean has the potential to become a key food and feed protein crop in many areas of the world. The presence of tannins in its seed coat has limited the deployment of this crop as feed and food. The expression of either of the two recessive genes, zt1 and zt2, causes a great reduction of tannins from the seed coat and results in a white flower phenotype. Molecular markers linked to these loci are fundamental tools for speeding up the breeding of low‐tannin varieties. The main aim of this study was to develop and validate a robust molecular marker linked to the zt2 locus. We used 176 recombinant inbred lines of the Disco/2 × ILB 938/2 cross at F₆ and genotyped those using 257 SNP (single nucleotide polymorphism) markers. An SNP marker associated with zt2 locus was found on faba bean chromosome 3 and was used to develop a high‐throughput low‐cost KASP (kompetitive allele‐specific PCR) marker. The KASP marker can successfully discriminate low‐tannin faba beans carrying zt2 from those carrying zt1 and wild‐type alleles.

中文翻译：

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蚕豆（Vicia faba）zt2基因座稳健KASP标记的开发和验证

蚕豆有潜力成为世界许多地区重要的粮食和饲料蛋白质作物。种皮中单宁的存在限制了该作物作为饲料和食品的应用。两个隐性基因zt1和zt2的表达导致种皮中单宁的大量减少，并导致白花表型。与这些基因座相关的分子标记是加快低单宁品种育种的基本工具。这项研究的主要目的是开发和验证与zt2基因座相关的强大分子标记。我们在F ₆使用了Disco / 2×ILB 938/2杂交的176个重组自交系并使用257个SNP（单核苷酸多态性）标记对它们进行基因分型。在蚕豆第3号染色体上发现了与zt2基因座相关的SNP标记，用于开发高通量低成本KASP（竞争性等位基因特异性PCR）标记。KASP标记可以成功地将携带zt2的低单宁蚕豆与携带zt1和野生型等位基因的蚕豆区分开。