Soybean is a special crop that can utilize N₂ in the air via symbioses with Rhizobium spp. The formation of effective nodules is a complex process in which nodulation outer proteins (Nops) are determinants of establishment of a symbiotic relationship. We constructed a Sinorhizobium fredii HH103ΩnopB mutant. A nodulation test showed that the mutant had a negative effect on the Suinong14, ZYD00006, Dongnong594 and Charleston soybean lines. Recombinant inbred soybean lines were independently inoculated with the mutant and wild‐type strains, and five and four quantitative trait loci (QTLs) were identified by analysing the nodule number (NN) and nodule dry weight (NDW), respectively. We chose one QTL that overlapped with other studies and a novel QTL identified in our study and selected six candidate genes for further analysis. The qRT‐PCR analysis showed that only changes in Glyma.17G166200 expression depended on NopB. Further analysis showed that Glyma.17G166200 encoded a protein with a D‐glucose‐binding domain and a serine‐threonine/tyrosine protein kinase catalytic domain that was involved in the abscisic acid (ABA) pathway.

中文翻译：

---

QTL定位和基因挖掘以鉴定与费氏中华根瘤菌HH103 NopB相关的大豆（Glycine max）基因

大豆是一种特殊的作物，可以通过与根瘤菌的共生作用在空气中利用N ₂。有效结节的形成是一个复杂的过程，其中结节外部蛋白（Nops）是建立共生关系的决定因素。我们构建了费氏中华根瘤菌HH103Ω NOPB突变体。结瘤试验表明，该突变体对Suinong14，ZYD00006，Dongnong594和Charleston大豆品系具有负面影响。将重组近交大豆系分别接种突变株和野生型菌株，并分别通过分析根瘤数（NN）和根瘤干重（NDW）鉴定出五个和四个定量性状位点（QTL）。我们选择了一个与其他研究重叠的QTL，并在研究中确定了一个新颖的QTL，并选择了六个候选基因进行进一步分析。qRT-PCR分析表明，只有Glyma.17G166200表达的变化取决于NopB。进一步分析表明，Glyma.17G166200 编码具有D-葡萄糖结合结构域和丝氨酸-苏氨酸/酪氨酸蛋白激酶催化结构域的蛋白质，该结构域涉及脱落酸（ABA）途径。