Pyramiding Asian soybean rust (ASR) resistance (Rpp) genes in a single genotype has been shown to increase ASR resistance in soybean. However, it remains unclear which combinations of Rpp genes are superior. Therefore, here, we developed six new Rpp‐pyramided lines carrying different combinations of Rpp genes and evaluated their resistance against 13 Bangladeshi rust (Phakopsora pachyrhizi) isolates (BdRPs) alongside seven previously developed Rpp‐pyramided lines. We found that lines carrying one, two and three Rpp genes had high ASR resistance without sporulation in 13.8%, 35.2% and 73.1% of the assays, respectively. Among the new lines that were developed, those with Rpp3 + Rpp4 and Rpp3 + Rpp4 + Rpp5 had high levels of ASR resistance, while the line containing Rpp2 + Rpp4 + Rpp5 showed immunity phenotype at two weeks after inoculation by the BdRP‐22 infection. Thus, pyramiding larger numbers of Rpp genes confers soybean with a higher level of resistance to ASR pathogens and can produce an immunity phenotype at two weeks after inoculation.

中文翻译：

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金字塔化三个抗锈基因赋予大豆高水平的抗性（Glycine max）

已显示单一基因型的金字塔型亚洲大豆锈病（ASR）抗性（Rpp）基因可增加大豆中的ASR抗性。但是，尚不清楚哪种Rpp基因组合是更好的。因此，在这里，我们开发了六种带有不同Rpp基因组合的Rpp金字塔新品系，并评估了它们对13种孟加拉锈病（Phakopsora pachyrhizi）分离株（BdRPs）的抗性以及七种先前开发的Rpp酰胺化品系。我们发现载有1、2和3 Rpp的线这些基因分别在13.8％，35.2％和73.1％的检测中具有较高的ASR抗药性且无孢子形成。在已开发的新品系中，具有Rpp3  +  Rpp4和Rpp3  +  Rpp4  +  Rpp5的品系具有较高的ASR抗性，而包含Rpp2  +  Rpp4  +  Rpp5的品系在接种BdRP-22感染后两周显示免疫表型。因此，金字塔状的Rpp基因数量更多，使大豆对ASR病原体具有更高的抗性水平，并可以在接种后两周产生免疫表型。