Direct seeding is increasingly being practiced in rice cultivation areas because it saves labour and reduces cost of production and management. In this study, four japonica‐type breeding lines were developed from a cross of a japonica cultivar and an AG‐tolerant donor, KHO, using marker‐assisted selection. Validation of the target genes AG1 and AG2 in the selected lines was examined by PCR analysis and the background recovery rate by the recurrent parent was confirmed by genotyping using a 6K SNP chip. Compared to the recurrent, the survival rates of the selected lines were improved in a range from 33% to 115% in three conditions; in particular, the survival rate was increased in more than twice that of the recurrent parent under the hypoxic condition. The main agronomic trait, yield and quality‐related traits were similar to those of the recurrent parent. The use of these lines will contribute to the expansion of the cultivation area due to the proven economic benefits of direct seeding in Korea.

中文翻译：

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标记辅助育种提高粳稻无氧萌发能力

在水稻种植地区越来越多地采用直接播种，因为这样可以节省劳动力并降低生产和管理成本。在这项研究中，使用标记辅助选择技术，从一个粳稻品种和一个耐AG的供体KHO的杂交中开发了四个粳型育种系。靶基因AG1和AG2的验证通过PCR分析检查所选品系中的H2O3，并通过使用6K SNP芯片进行基因分型来确认复发亲本的背景回收率。与复发相比，在三种情况下，所选品系的存活率提高了33％至115％。特别是在缺氧条件下，成活率增加了复发父母的两倍以上。主要农艺性状，产量和品质相关性状与轮回亲本相似。由于在韩国直接播种已证明具有经济效益，因此使用这些品种将有助于扩大种植面积。