Vitamin E (VE) is an important nutritional trait in rice grains. In order to dissect the genetic basis underlying VE content, a recombinant inbred lines population derived from 'Zhenshan 97B' and 'Nanyangzhan' was used for quantitative trait locus (QTL) analysis. Totally, 29 QTLs for six VE traits were identified in 2 consecutive years. Among those, five QTLs repeatedly detected in two years formed a cluster on chromosome 2, which was responsible for all five VE isomers. OsγTMT, the gene encoding γ‐tocopherol methyltransferase in rice, was located to the same region and treated as the candidate gene. Sequence analysis of alleles from two parents revealed many polymorphisms, including 19 single nucleotide polymorphisms (SNPs) and two insert/deletions (Indels) in the promoter region, two nonsynonymous SNPs in exons, and 25 SNPs and an Indel in introns. Besides, a QTL for δ‐tocotrienol and two QTLs for α‐tocopherol were repeatedly detected on chromosome 5 and 8, respectively, all three regions carrying no homologous genes involved in VE biosynthesis. These results could be useful in development of rice lines displaying desirable VE content.

中文翻译：

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利用“ Zhenshan97B”和“ Nanyangzhan”杂交后代的重组自交系遗传剖析水稻籽粒中维生素E含量

维生素E（VE）是米粒中重要的营养性状。为了剖析VE含量的遗传基础，使用了来自'Zhenshan 97B'和'Nanyangzhan'的重组自交系种群进行数量性状基因座（QTL）分析。连续2年共鉴定了29个QTL，涉及6个VE特性。其中，在两年内反复检测到的五个QTL在2号染色体上形成了一个簇，该簇负责所有五个VE异构体。OsγTMT水稻中编码γ-生育酚甲基转移酶的基因位于同一区域，被视为候选基因。来自两个亲本的等位基因的序列分析揭示了许多多态性，包括启动子区域中的19个单核苷酸多态性（SNP）和两个插入/缺失（Indels），外显子中的两个非同义SNPs和内含子中的25个SNP和一个Indel。此外，分别在5号和8号染色体上分别检测到δ-生育三烯酚的QTL和两个α-生育酚的QTL，这三个区域均不携带参与VE生物合成的同源基因。这些结果可能对显示期望的VE含量的水稻品系有用。