Cellulose is one of the main chemical component of bast fibre in jute. However, quantitative trait loci (QTL) for bast fibre cellulose content remains elusive. In this study, we identified 846 new SSR markers from 70,792 unigenes in the NCBI and validated them in a panel of 24 diverse jute accessions. Of 846 SSRs, 748 (88.41%) were successfully amplified, and 585 (69.14%) showed polymorphisms, implying that these are high‐quality SSRs. Furthermore, 585 SSRs along with 5,074 polymorphic SLAF (specific locus amplified fragment) and 173 InDel markers were used to reconstruct a high‐dense linkage map in a recombinant inbred population with 104 F₈ lines. Totally, 835 markers were successfully mapped to a whole length of 604.5 cM with a mean distance of 2.84 cM between adjacent markers. Furthermore, five QTLs for bast fibre cellulose were identified. One major QTL (qBFC1‐1) was stable in 2 years and explained average phenotypic variance with 14.34%. These results may be useful for developing enhanced bast fibre quality in white jute through marker‐assisted selection (MAS) breeding.

中文翻译：

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评估新开发的SSR标记和鉴定白麻中韧皮纤维纤维素的数量性状基因座

纤维素是黄麻韧皮纤维的主要化学成分之一。然而，韧皮纤维纤维素含量的定量性状位点（QTL）仍然难以捉摸。在这项研究中，我们从NCBI的70,792个单基因中鉴定了846个新的SSR标记，并在24种不同的黄麻种质中进行了验证。在846个SSR中，成功扩增了748个（88.41％），并且有585个（69.14％）具有多态性，这表明它们是高质量的SSR。此外，在具有104 F ₈的重组近交种群中，使用585个SSR和5,074个多态性SLAF（特定基因座扩增片段）和173个InDel标记物来构建高密度连锁图谱。线。总共成功地将835个标记物映射到604.5 cM的全长，相邻标记物之间的平均距离为2.84 cM。此外，鉴定了五个韧皮纤维纤维素的QTL。一个主要的QTL（qBFC1-1）在2年内保持稳定，并解释了平均表型差异为14.34％。这些结果可能有助于通过标记辅助选择（MAS）育种提高白麻中的韧皮纤维质量。