ABSTRACT The accumulation of multi-hydrolytic enzyme through anaerobic co-digestion of waste-activated sludge (WAS) and food waste (FW) was studied by regulating temperature, pH and the mass ratio of FW to WAS (F/W). Experimental results showed that temperature had a profound effect on the activity of the enzyme and the most suitable temperatures for the accumulation of amylase and protease were 37°C and 50°C, respectively. The highest activity of amylase and protease accumulated reached 10.29 and 19.23 U/mL at an F/W ratio of 2:1. The addition of anaerobic co-digestion solution enriching protease and amylase had positive effects on the hydrolysis of WAS. In addition, the Illumina high-throughput sequencing demonstrated that the bacterial diversity decreased, but the bacterial abundance increased during the co-digestion process of WAS and FW. The predominant strains for secreting amylase were Lactobacillus and Clostridium-sensu-strito-1, and Aeromonas was the dominant strain for secreting protease. GRAPHICAL ABSTRACT

中文翻译：

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餐厨垃圾与废弃活性污泥厌氧共消化多水解酶积累及微生物群落结构

摘要 通过调节温度、pH 值和 FW 与 WAS 的质量比 (F/W)，研究了通过废物活性污泥 (WAS) 和食物垃圾 (FW) 厌氧共同消化多水解酶的积累。实验结果表明，温度对酶的活性有很大的影响，淀粉酶和蛋白酶最适宜积累的温度分别为37℃和50℃。在 F/W 比为 2:1 时，积累的淀粉酶和蛋白酶的最高活性分别达到 10.29 和 19.23 U/mL。添加富含蛋白酶和淀粉酶的厌氧共消化溶液对WAS的水解有积极影响。此外，Illumina高通量测序表明，在WAS和FW共同消化过程中，细菌多样性下降，但细菌丰度增加。分泌淀粉酶的优势菌株是乳酸杆菌和梭菌-sensu-strito-1，气单胞菌是分泌蛋白酶的优势菌株。图形概要