Strain QCG of the aerobic bacteria Bacillus cereus is capable of producing 1-naphthol from naphthalene, this strain was first isolated and characterized in this study. Strain QCG was mutagenized to enhance 1-naphthol production, using atmospheric and room temperature plasma (ARTP) technology. Then, a microbial clone screening system was used to accelerate the operation. Meanwhile, a novel color-mediated high-throughput screening using 4-aminoantipyrine was performed to screen mutants. The optimal mutant strain QCG4 produced 19.58±0.34 mg∙L⁻¹ 1-naphthol from naphthalene that was 47.32% higher than that of the original strain (13.29±0.28 mg∙L⁻¹). In addition, the optimal conditions for 1-naphthol production via whole-cell catalysis of strain QCG4 were determined to be an OD₆₀₀ of 40,150 mg∙L⁻¹ naphthalene, and 7.5% dimethyl formamide as a co-solvent at pH 7.5 and 26°C for 3 h, resulting in 41.18±0.12 mg-L⁻¹ 1-naphthol, i.e., the mutant strain produces a 2.1-fold higher yield compared to the original strain.

有氧细菌蜡样芽胞杆菌的QCG菌株能够从萘中产生1-萘酚，该菌株首先被分离并进行了表征。使用大气和室温等离子体（ARTP）技术诱变QCG菌株以增强1-萘酚的产量。然后，使用微生物克隆筛选系统来加速操作。同时，使用4-氨基安替比林进行了新颖的颜色介导的高通量筛选，以筛选突变体。最佳突变株QCG4由萘产生19.58±0.34 mg∙L ^-1 1-萘酚，比原始菌株（13.29±0.28 mg∙L ^-1）高47.32％。）。此外，确定通过QCG4菌株全细胞催化生产1-萘酚的最佳条件是OD ₆₀₀为40,150 mg∙L ^-1萘和7.5％二甲基甲酰胺作为助溶剂，pH 7.5和26 °C持续3 h，得到41.18±0.12 mg-L ^-1 1-萘酚，即与原始菌株相比，该突变菌株的产量高2.1倍。