Objectives

There is a controversy about the D* and f values of intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) for mid- and long-term efficacy monitoring of tumor blood perfusion. To monitor the antitumor efficacy of the F/A-PLGA@DOX/SPIO nanosystem via IVIM-DWI and to explore the value of parameters pseudo-diffusion (D*) and fraction of pseudo-diffusion (f) for evaluating therapeutic effect in non-small cell lung cancer (NSCLC).

Materials and methods

Thirty-six A549 tumor-bearing mice were divided randomly into three groups (each n = 12). Group 1 (G1) was injected with saline (the control group). Group 2 (G2) and group 3(G3) were injected with DOX and F/A-PLGA@DOX/SPIO, respectively. Each group underwent IVIM-DWI scanning at baseline and 3, 14, 21, and 28 days after treatment. D* and f values were derived using GE AW 4.5 post-processing station. All mice were sacrificed for pathological examination.

Results

The D* value of all three groups showed an upward trend, with the highest increase in G1 and the lowest in G3. Conversely, the f value of all groups trended to decrease within 7 days, of which G3 showed the most significant decline. Immunohistochemical staining revealed that vascular endothelial growth factor (VEGF)-positive staining rate and the microvessel density (MVD) of the tumors in G3 were significantly lower than those of the other groups (P < 0.05). The D* and f values were significantly and positively correlated to CD31 (r = 0.654, P < 0.001; r = 0.712, P < 0.001) and VEGF (r = 0.694, P < 0.001; r = 0.664, P < 0.001).

Conclusion

IVIM-DWI-derived parameters D* and f are valuable indicators for the evaluation of the antitumor microcirculation changes of multifunctional nanosystem.

中文翻译：

---

使用多功能纳米系统执行IVIM-DWI，以评估抗肿瘤微循环变化。

目标

关于体素内非相干运动扩散加权成像（IVIM-DWI）的D *和f值在肿瘤血液灌注的中长期监测中存在争议。通过IVIM-DWI监测F / A-PLGA @ DOX / SPIO纳米系统的抗肿瘤功效，并探讨伪扩散参数（D *）和伪扩散分数（f）的价值，以评估非药物治疗的疗效。 -小细胞肺癌（NSCLC）。

材料和方法

将36只A549荷瘤小鼠随机分为三组（每组n  = 12）。第1组（G1）注射生理盐水（对照组）。第2组（G2）和第3组（G3）分别注射DOX和F / A-PLGA @ DOX / SPIO。每组均在基线以及治疗后第3、14、21和28天接受IVIM-DWI扫描。D *和f值是使用GE AW 4.5后处理站得出的。处死所有小鼠以进行病理检查。

结果

三组的D *值均呈上升趋势，G1的增幅最高，G3的增幅最低。相反，所有组的f值在7天内都有下降的趋势，其中G3下降最为明显。免疫组织化学染色显示，G3组肿瘤血管内皮生长因子（VEGF）阳性染色率和微血管密度（MVD）明显低于其他组（P  <0.05）。的d *和˚F值显著和正相关CD31（- [R  = 0.654，P  <0.001; - [R  = 0.712，P  <0.001）和VEGF（ř = 0.694，P  <0.001；r  = 0.664，P  <0.001）。

结论

IVIM-DWI衍生的参数D *和f是评估多功能纳米系统抗肿瘤微循环变化的有价值指标。