Fluorometric and electrochemical dual-mode nanoprobe for tetracycline by using a nanocomposite prepared from carbon nitride quantum dots and silver nanoparticles,Microchimica Acta

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Fluorometric and electrochemical dual-mode nanoprobe for tetracycline by using a nanocomposite prepared from carbon nitride quantum dots and silver nanoparticles
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-01-01 , DOI: 10.1007/s00604-019-3828-4
Xuetao Hu ₁ , Yiwei Xu ₁ , Xueping Cui ₁ , Wenting Li ₁ , Xiaowei Huang ₁ , Zhihua Li ₁ , Jiyong Shi ₁ , Xiaobo Zou ₁

Affiliation

A fluorometric and electrochemical dual-mode method is described for sensitive and specific detection of tetracycline (Tc). A novel nanoprobe was designed that is making use of a Tc-specific aptamer (apta), carbon nitride quantum dots (CNQDs) and silver nanoparticles (AgNPs). The aptamer was linked to the CNQDs which then were used as templates to synthesize the apta-CNQD@AgNP nanocomposites. The blue fluorescence of the nanocomposites (with excitation/emission maxima at 365/440 nm) is quenched. The addition of Tc leads to fluorescence recovery and a decrease in the electroconductivity of a gold electrode modified with apta-CNQD@AgNPs. The fluorometric method has a linear response in the 0.1 μM - 10 mM Tc concentration range and a 15 nM detection limit. The amperometric method (best performed at a working voltage of 0.21 V vs. Ag/AgCl) has a linear response in the 1 nM to 0.1 mM Tc concentration range and a 0.26 nM detection limit. Recoveries of Tc from spiked milk samples were comparable to data obtained by HPLC. Graphical abstract A fluorometric and electrochemical dual-mode nanoprobe (apta-CNQD@AgNPs) was prepared from aptamer (apta), carbon nitride quantum dots (CNQDs) and silver nanoparticles (AgNPs). The nanoprobe can be used for determination of tetracycline (Tc) based on fluorescence recovery of apta-CNQD@AgNPs and a decrease in the electroconductivity of a gold electrode modified with apta-CNQD@AgNPs.

中文翻译：

使用由氮化碳量子点和银纳米粒子制备的纳米复合材料制备四环素的荧光和电化学双模式纳米探针

描述了一种用于敏感和特异检测四环素 (Tc) 的荧光和电化学双模式方法。设计了一种新型纳米探针，它利用了 Tc 特异性适体 (apta)、氮化碳量子点 (CNQD) 和银纳米粒子 (AgNP)。适体与 CNQD 相连，然后用作模板合成适体-CNQD@AgNP 纳米复合材料。纳米复合材料的蓝色荧光（激发/发射最大值在 365/440 nm）被淬灭。Tc 的添加导致荧光恢复和用 apta-CNQD@AgNPs 修饰的金电极的电导率降低。荧光法在 0.1 μM - 10 mM Tc 浓度范围内具有线性响应，检测限为 15 nM。电流法（最好在 0.21 V 的工作电压与 Ag/AgCl) 在 1 nM 至 0.1 mM Tc 浓度范围内具有线性响应，检测限为 0.26 nM。从加标牛奶样品中回收的 Tc 与 HPLC 获得的数据相当。图形摘要从适体 (apta)、氮化碳量子点 (CNQDs) 和银纳米粒子 (AgNPs) 制备了荧光和电化学双模式纳米探针 (apta-CNQD@AgNPs)。基于apta-CNQD@AgNPs的荧光恢复和用apta-CNQD@AgNPs修饰的金电极的电导率降低，纳米探针可用于测定四环素（Tc）。图形摘要从适体 (apta)、氮化碳量子点 (CNQDs) 和银纳米粒子 (AgNPs) 制备了荧光和电化学双模式纳米探针 (apta-CNQD@AgNPs)。基于apta-CNQD@AgNPs的荧光恢复和用apta-CNQD@AgNPs修饰的金电极的电导率降低，纳米探针可用于测定四环素（Tc）。图形摘要从适体 (apta)、氮化碳量子点 (CNQDs) 和银纳米粒子 (AgNPs) 制备了荧光和电化学双模式纳米探针 (apta-CNQD@AgNPs)。基于apta-CNQD@AgNPs的荧光恢复和用apta-CNQD@AgNPs修饰的金电极的电导率降低，纳米探针可用于测定四环素（Tc）。

更新日期：2020-01-01

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