Abstract

Protein and peptide phosphorylation regulate numerous pathological processes, however, their characterization is challenging due to their low abundance and transient nature. Therefore, nanomaterials are being developed to address this demanding task. In particular, carbon nanostructures are attracting interest as scaffolds for functional nanocomposites, yet only isolated studies exist on the topic, and little is known on the effect of nanocarbon morphology on templating nanocomposites. In this work, we compared oxidized carbon nanotubes, graphene oxide, oxidized carbon nanohorns and oxidized graphitized carbon black, as scaffolds for magnetized nanocomposites. The nanomaterials were extensively characterized with experimental and in silico techniques. Next, they were applied to phosphopeptide enrichment from cancer cell lysates for NanoHPLC-MS/MS, with selectivity as high as nearly 90% and several-thousand identification hits. Overall, new insights emerged for the understanding and the design of nanocomposites for phosphoproteomics.

中文翻译：

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用于磷酸化蛋白质组学的碳纳米结构形态模板纳米复合材料

摘要

蛋白质和肽的磷酸化调节许多病理过程，但是，由于其低丰度和短暂的特性，其表征具有挑战性。因此，正在开发纳米材料来解决这一艰巨的任务。特别地，碳纳米结构作为功能性纳米复合材料的支架引起了人们的兴趣，但是只有单独的研究存在，而且关于纳米碳形态对模板化纳米复合物的影响知之甚少。在这项工作中，我们比较了氧化碳纳米管，氧化石墨烯，氧化碳纳米角和氧化石墨化炭黑作为磁化纳米复合材料的支架。通过实验和计算机模拟对纳米材料进行了广泛表征技术。接下来，将其应用于癌细胞裂解液中的磷酸肽富集，用于NanoHPLC-MS / MS，选择性高达近90％，鉴定命中率高达数千。总体而言，对于磷酸化蛋白质组学的纳米复合材料的理解和设计，出现了新的见解。