This paper is to demonstrate an efficient cell capture assay based on employing patterned highly vertical zinc oxide (ZnO) nanorods. Hydrophilic properties of ZnO nanorods, as well as nano topographic nature of the outer surface of cells were exploited as separation mechanism. MCF-7 and MDA-MB-231 breast cancer cell lines were captured on the patterned or not patterned ZnO nanorods. For these substrates, significantly improved capture efficiency was achieved by patterning the nanorods surfaces. The capture efficiency of MCF-7 cell species increased from 0 to over 84 % through the conversion of flat substrates to patterned nanorods surfaces. Additionally, efficiency promotion was observed by increasing the incubation time from 1 hour to 4 hours for the MCF-7 cells, resulting in maximum capture efficiency of 98.4% in the case of patterned nanorods. However, MDA-MB-231 cancer cells, as invasive late-stage cell species, were expectantly found to show lower efficiencies, maximum of 86%. Intensively and strongly adhered breast cancer cells to nanorods with formed lamellipodia have been reported for proposed ZnO nanostructured substrates. Interestingly, both types of breast cell species depicted different cell areas, offering various focal adhesions. This allows for selective separation of not only the breast cancer cells from the normal blood species but also these two cell types while retaining viability.

中文翻译：

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使用图案化 ZnO 纳米棒阵列有效捕获循环肿瘤细胞

本文旨在展示一种基于采用图案化高度垂直氧化锌 (ZnO) 纳米棒的高效细胞捕获分析。ZnO 纳米棒的亲水性以及细胞外表面的纳米形貌特性被用作分离机制。MCF-7 和 MDA-MB-231 乳腺癌细胞系被捕获在图案化或未图案化的 ZnO 纳米棒上。对于这些基板，通过对纳米棒表面进行图案化，显着提高了捕获效率。通过将平坦基底转化为图案化纳米棒表面，MCF-7 细胞种类的捕获效率从 0 增加到超过 84%。此外，通过将 MCF-7 细胞的孵育时间从 1 小时增加到 4 小时，观察到效率提升，在图案化纳米棒的情况下，最大捕获效率为 98.4%。然而，MDA-MB-231 癌细胞作为侵入性晚期细胞种类，预计显示出较低的效率，最高为 86%。据报道，对于所提出的 ZnO 纳米结构基材，乳腺癌细胞与具有形成的片状伪足的纳米棒紧密且牢固地粘附在一起。有趣的是，两种类型的乳腺细胞都描绘了不同的细胞区域，提供了不同的粘着斑。这不仅允许从正常血种中选择性分离乳腺癌细胞，还允许选择性分离这两种细胞类型，同时保持活力。有趣的是，两种类型的乳腺细胞都描绘了不同的细胞区域，提供了不同的粘着斑。这不仅允许从正常血种中选择性分离乳腺癌细胞，还允许选择性分离这两种细胞类型，同时保持活力。有趣的是，两种类型的乳腺细胞都描绘了不同的细胞区域，提供了不同的粘着斑。这不仅允许从正常血种中选择性分离乳腺癌细胞，还允许选择性分离这两种细胞类型，同时保持活力。