Simultaneous determination of sertraline, imipramine and alprazolam in human plasma samples using headspace solid phase microextraction based on a nanostructured polypyrrole fiber coupled to ion mobility spectrometry,Analytical Methods

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Simultaneous determination of sertraline, imipramine and alprazolam in human plasma samples using headspace solid phase microextraction based on a nanostructured polypyrrole fiber coupled to ion mobility spectrometry
Analytical Methods ( IF 2.7 ) Pub Date : 2020/01/03 , DOI: 10.1039/c9ay02001b
Erfan Barati _{1,

2,

3,

4,

5} , Naader Alizadeh _{1,

2,

3,

4,

5}

Affiliation

This paper describes the development of a simple, rapid, and sensitive method based on headspace solid-phase microextraction (HS-SPME) combined with ion mobility spectrometry (IMS) for the simultaneous determination of antidepressant drugs, sertraline (SER), imipramine (IMI) and alprazolam (ALP), in human plasma samples. IMS equipped with corona discharge (CD) ionization along with HS-SPME has been successfully applied for quantitative analysis without derivatization steps. A dodecylbenzenesulfonate doped polypyrrole coating was used as a fiber for HS-SPME. The effects of various parameters on microextraction efficiency, including extraction temperature, extraction time, ionic strength, salt concentration and pH of the sample, were also evaluated and optimized to obtain the best HS-SPME results. The HS-SPME-IMS method provided good repeatability (RSD ≤ 5%) for three replicate analyses and a linear calibration range between 0.3–5 μg mL⁻¹, 0.5–5 μg mL⁻¹ and 1–5 μg mL⁻¹ for sertraline, imipramine and alprazolam, respectively (R² > 0.99). The limits of detection for sertraline, imipramine and alprazolam were 0.08, 0.12 and 0.27 μg mL⁻¹, respectively. The proposed method was applied for determination of sertraline, imipramine and alprazolam in human plasma samples. The analytical procedure was validated by using percentage of recovery and was confirmed by the add-found method in human plasma samples. The analyte recoveries obtained were in the range of 93–97%. The proposed method provides several advantages such as high sensitivity, good repeatability, organic solvent-free, and being less time-consuming and relatively inexpensive as compared to HPLC.

中文翻译：

基于纳米结构聚吡咯纤维与离子迁移谱联用的顶空固相微萃取同时测定人血浆样品中的舍曲林，丙咪嗪和阿普唑仑

本文描述了一种基于顶空固相微萃取（HS-SPME）结合离子迁移谱（IMS）的同时测定抗抑郁药，舍曲林（SER）和丙咪嗪（IMI）的简单，快速，灵敏的方法的开发和血浆中的阿普唑仑（ALP）。配备电晕放电（CD）电离和HS-SPME的IMS已成功用于定量分析而无需衍生化步骤。十二烷基苯磺酸盐掺杂的聚吡咯涂层用作HS-SPME的纤维。还评估并优化了各种参数对微萃取效率的影响，包括萃取温度，萃取时间，离子强度，盐浓度和样品的pH，以获得最佳的HS-SPME结果。^-1，0.5-5微克毫升^-1和1-5微克毫升^-1用于舍曲林，丙咪嗪和阿普唑仑，分别为（ - [R ² > 0.99）。舍曲林，丙咪嗪和阿普唑仑的检出限为0.08、0.12和0.27μgmL^-1，分别。该方法可用于测定人血浆中的舍曲林，丙咪嗪和阿普唑仑。通过使用回收率百分比验证了该分析程序，并通过添加发现的方法在人血浆样品中进行了确认。获得的分析物回收率在93–97％的范围内。所提出的方法具有许多优点，例如灵敏度高，重复性好，无有机溶剂，与HPLC相比耗时少且相对便宜。

更新日期：2020-02-20

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