The analysis of single cysteine molecules with an aerolysin nanopore,Analyst

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The analysis of single cysteine molecules with an aerolysin nanopore
Analyst ( IF 3.6 ) Pub Date : 2019/12/16 , DOI: 10.1039/c9an01965k
Bo Yuan _{1,

2,

3,

4,

5} , Shuang Li _{1,

2,

3,

4} , Yi-Lun Ying _{1,

2,

3,

4,

5} , Yi-Tao Long _{4,

5,

6,

7}

Affiliation

Biological nanopore technology has the advantages of high selectivity and high reproducibility for characterizing single biomolecules. However, it is challenging to achieve protein sequencing owing to the heterogeneous charge distributions of the protein and the small structural difference from each amino acid. Here, we took the inherent electrochemically confined sensing interface of the aerolysin nanopore to enhance its interaction with single amino acids. The results showed that single cysteine molecules, a highly reactive amino acid in aging and neurodegenerative diseases, could be captured and monitored by an aerolysin nanopore as it produced distinctive current blockages with a prolonged statistical duration of 0.11 ± 0.02 ms at +120 mV. This is the first report of the detection of a single amino acid molecule by a biological nanopore directly without any modification and labelling. This study facilitates the direct detection of single amino acids by regulating the characteristic interaction between the single amino acids and the designed sensing interface of aerolysin nanopores.

中文翻译：

气溶素纳米孔对单个半胱氨酸分子的分析

生物纳米孔技术具有表征单个生物分子的高选择性和高重现性的优点。然而，由于蛋白质的异质电荷分布和与每个氨基酸的较小结构差异，实现蛋白质测序具有挑战性。在这里，我们采用了溶血素纳米孔固有的电化学限制的传感界面，以增强其与单个氨基酸的相互作用。结果表明，一个半胱氨酸分子是衰老和神经退行性疾病中的一种高反应性氨基酸，可以通过aerolysin纳米孔捕获和监测，因为它在+120 mV时会产生明显的电流阻滞，并具有0.11±0.02 ms的延长统计持续时间。这是直接通过生物纳米孔检测单个氨基酸分子而没有任何修饰和标记的报告。这项研究通过调节单个氨基酸与溶血素纳米孔的设计传感界面之间的相互作用，促进了单个氨基酸的直接检测。

更新日期：2020-02-17

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