4D hydrogel for dynamic cell culture with orthogonal, wavelength-dependent mechanical and biochemical cues†,Materials Horizons

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4D hydrogel for dynamic cell culture with orthogonal, wavelength-dependent mechanical and biochemical cues†
Materials Horizons ( IF 12.2 ) Pub Date : 2019-07-22 , DOI: 10.1039/c9mh00665f
Yijun Zheng _{1,

2,

3,

4} , Mitchell Kim Liong Han _{1,

2,

3,

4} , Qiyang Jiang _{1,

2,

3,

4,

5} , Bin Li _{1,

2,

3,

4} , Jun Feng _{1,

2,

3,

4,

5} , Aránzazu del Campo _{1,

2,

3,

4,

5}

Affiliation

Cooperative action of biochemical and biomechanical signals regulates the interactions between cells and the supporting matrix in natural tissues. Herein, we describe a hydrogel for 4D cell culture which allows user-defined stiffening of the cellular environment and presentation of bioadhesive cues in an orthogonal manner using light of different wavelengths. Stiffening of the gel is initiated by VIS light, while activation of the biochemical function is triggered by UV light. We demonstrate the versatility of this system by triggering, directing and/or hindering cell migration from spheroids based on photoactivated stiffening or integrin-binding to the hydrogels. This material allows in situ and independent manipulation of the physicochemical cues in the cellular microenvironment in vitro, and could eventually be extended to in vivo.

中文翻译：

用于动态细胞培养的4D水凝胶，具有正交的，与波长有关的机械和生化线索†

生化和生物力学信号的协同作用调节自然组织中细胞与支持基质之间的相互作用。在这里，我们描述了一种用于4D细胞培养的水凝胶，该凝胶允许用户定义的细胞环境变硬以及使用不同波长的光以正交方式呈现生物粘附提示。凝胶的硬化是由可见光引发的，而生化功能的激活是由紫外线引发的。我们通过基于光活化的硬化或整联蛋白结合到水凝胶上的球体触发，指导和/或阻止细胞迁移证明了该系统的多功能性。这种材料允许在体外对细胞微环境中的理化线索进行原位和独立操作，并最终可以推广到体内。

更新日期：2020-01-04

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