Abstract The present study was conducted to evaluate the efficacy of a new technique for removal of campylobacters from naturally contaminated free-range and broiler chicken carcasses. The carcasses, obtained from commercial processing plants after evisceration, were immersed in 0.1% cetylpyridinium chloride (CPC) or 0.01% sodium hypochlorite (NaOCl) and exposed to a vacuum of −0.02 MPa to remove air from feather. The carcasses were then immersed in a disinfectant solution in a steel container and subjected to ultrasonication with a water resonance system (WRS) at 130 kHz. Tap water was also used instead of the disinfectant, and other carcasses were immersed in each chemical alone. The Campylobacter count on breast and back skin was determined by the most-probable-number (MPN) method. This new treatment using CPC and NaOCl significantly reduced the Campylobacter count on back and breast skin. Treatment with 0.1% CPC combined with vacuum and ultrasonication with the WRS was the most effective, achieving a Campylobacter reduction of 1.36–1.64 log10 MPN/10 g and 0.94–1.16 log10 MPN/10 g on free-range and broiler chicken carcasses, respectively. Scanning electron microscopy (SEM) confirmed that this treatment successfully removed debris from breast skin in comparison to untreated skin. These results suggest that the use of sanitizers employing a combination of vacuum and ultrasonication with a WRS is highly effective for reducing the count of campylobacters on chicken carcasses.

中文翻译：

---

化学处理结合真空和超声波处理与水共振系统减少自然污染鸡尸体上的弯曲杆菌的评价

摘要 本研究旨在评估从自然污染的散养鸡和肉鸡屠体中去除弯曲杆菌的新技术的效果。将内脏后从商业加工厂获得的胴体浸入 0.1% 氯化十六烷基吡啶 (CPC) 或 0.01% 次氯酸钠 (NaOCl) 中，并暴露在 -0.02 MPa 的真空中以去除羽毛中的空气。然后将尸体浸入钢制容器中的消毒剂溶液中，并使用水共振系统 (WRS) 在 130 kHz 下进行超声波处理。还使用自来水代替消毒剂，并将其他尸体单独浸入每种化学品中。乳房和背部皮肤上的弯曲杆菌计数由最可能数 (MPN) 方法确定。这种使用 CPC 和 NaOCl 的新疗法显着减少了背部和胸部皮肤上的弯曲杆菌数量。使用 0.1% CPC 结合真空和 WRS 超声处理是最有效的，在散养和肉鸡胴体上分别实现 1.36–1.64 log10 MPN/10 g 和 0.94–1.16 log10 MPN/10 g 弯曲杆菌减少. 扫描电子显微镜 (SEM) 证实，与未经处理的皮肤相比，这种处理成功地去除了乳房皮肤上的碎屑。这些结果表明，使用真空和超声波处理与 WRS 相结合的消毒剂对于减少鸡尸体上弯曲杆菌的数量非常有效。分别在散养和肉鸡屠体上实现弯曲杆菌减少 1.36–1.64 log10 MPN/10 g 和 0.94–1.16 log10 MPN/10 g。扫描电子显微镜 (SEM) 证实，与未经处理的皮肤相比，这种处理成功地去除了乳房皮肤上的碎屑。这些结果表明，使用真空和超声波处理与 WRS 相结合的消毒剂对于减少鸡尸体上弯曲杆菌的数量非常有效。分别在散养和肉鸡屠体上实现弯曲杆菌减少 1.36–1.64 log10 MPN/10 g 和 0.94–1.16 log10 MPN/10 g。扫描电子显微镜 (SEM) 证实，与未经处理的皮肤相比，这种处理成功地去除了乳房皮肤上的碎屑。这些结果表明，使用真空和超声波处理与 WRS 相结合的消毒剂对于减少鸡尸体上弯曲杆菌的数量非常有效。