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Manganese oxide doped carbon dots for temperature-responsive biosensing and target bioimaging
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.aca.2020.01.001
Chunmei Li 1 , Zhaojian Qin 1 , Maonan Wang 1 , Weiwei Liu 1 , Hui Jiang 1 , Xuemei Wang 1
Affiliation  

We report on the synthesis of manganese oxide doped CDs (MnOx-CDs) by a hydrothermal strategy using manganese (III) acetylacetonate (Mn(III) (C5H7O2)3) as the only raw materials. The MnOx-CDs exhibit water solubility, favorable biocompatibility, low cytotoxicity, and show blue fluorescence with excitation/emission maxima at 326/442 nm with a quantum yield of 11.3%, allowing efficient cellular imaging. The MnOx-CDs have a reversible temperature-sensitive fluorescent property in vitro within 10-60 °C, which can also be used as a sensitive thermometer in living cells. By a scratch assay, the MnOx-CDs can restrain the migration of HepG2 cancer cells, which make the MnOx-CDs be attractive candidates for liver cancer adjuvant treatment. Besides, the fluorescence of the MnOx-CDs is quenched in the presence of Fe3+ due to the formation of a nonfluorescent MnOx-CDs-Fe3+ complex between oxygen-containing groups on the surface of MnOx-CDs and Fe3+, and the quenched fluorescence of MnOx-CDs can be turn-on by dissociation of MnOx-CDs-Fe3+ complexes by biothiols including L-cysteine, homocysteine and glutathione. Therefore, the Fe3+ and biothiols can be sequentially detected with high reliability and accuracy via exploiting the on-off-on nanosensor at room temperature, respectively. Further application to detection biothiols in human serum indicated that the probe was practicality and feasibility in medical field.

中文翻译:

用于温度响应生物传感和目标生物成像的氧化锰掺杂碳点

我们报告了使用乙酰丙酮锰 (III) (Mn(III) (C5H7O2)3) 作为唯一原材料通过水热策略合成氧化锰掺杂的 CDs (MnOx-CDs)。MnOx-CDs 具有水溶性、良好的生物相容性、低细胞毒性,并显示蓝色荧光,激发/发射最大值为 326/442 nm,量子产率为 11.3%,可实现高效的细胞成像。MnOx-CDs 在体外 10-60 °C 内具有可逆的温度敏感荧光特性,也可用作活细胞中的敏感温度计。通过划痕试验,MnOx-CDs 可以抑制 HepG2 癌细胞的迁移,这使得 MnOx-CDs 成为肝癌辅助治疗的有吸引力的候选者。除了,由于在 MnOx-CDs 和 Fe3+ 表面的含氧基团之间形成了非荧光的 MnOx-CDs-Fe3+ 络合物,MnOx-CDs 的荧光在 Fe3+ 存在下被猝灭,并且 MnOx-CDs 的荧光猝灭可以通过生物硫醇(包括 L-半胱氨酸、高半胱氨酸和谷胱甘肽)解离 MnOx-CDs-Fe3+ 复合物来开启。因此,通过在室温下利用开关纳米传感器,可以分别以高可靠性和准确性依次检测 Fe3+ 和生物硫醇。进一步应用于人血清中生物硫醇的检测表明该探针在医学领域具有实用性和可行性。MnOx-CDs 的猝灭荧光可以通过包括 L-半胱氨酸、高半胱氨酸和谷胱甘肽在内的生物硫醇解离 MnOx-CDs-Fe3+ 复合物来开启。因此,通过在室温下利用开关纳米传感器,可以分别以高可靠性和准确性依次检测 Fe3+ 和生物硫醇。进一步应用于人血清中生物硫醇的检测表明该探针在医学领域具有实用性和可行性。MnOx-CDs 的猝灭荧光可以通过包括 L-半胱氨酸、高半胱氨酸和谷胱甘肽在内的生物硫醇解离 MnOx-CDs-Fe3+ 复合物来开启。因此,通过在室温下利用开关纳米传感器,可以分别以高可靠性和准确性依次检测 Fe3+ 和生物硫醇。进一步应用于人血清中生物硫醇的检测表明该探针在医学领域具有实用性和可行性。
更新日期:2020-04-01
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