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A smartphone-integrated paper sensing system for fluorescent and colorimetric dual-channel detection of foodborne pathogenic bacteria.
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2020-01-03 , DOI: 10.1007/s00216-019-02208-z
Chengnan Wang 1 , Xia Gao 1 , Shuo Wang 2 , Yaqing Liu 1, 3
Affiliation  

Infections caused by foodborne microorganisms are a great threat to the global environment and public healthcare today. Thus, rapid, portable and sensitive assays that can realize the identification of foodborne bacteria are highly desired. In this study, a smart fluorescent and colorimetric dual-readout sensing system has been established for simple and rapid E. coli determination by utilizing the Cu2+-triggered oxidation of o-phenylenediamine (OPD). Initially, Cu2+ can oxidize OPD to OPDox, resulting in an orange-yellow fluorescence and visible pale-yellow color. However, E. coli can effectively reduce Cu2+ into Cu+, inhibiting the Cu2+-triggered oxidation of OPD to OPDox. Consequently, the introduction of E. coli can turn off both the fluorescence and the UV-vis absorbance signals of the OPD-Cu2+ system, illustrating an original mechanism for fluorescent and colorimetric dual-channel detection of E. coli. Moreover, a filter paper-based visual sensor was built and coupled with OPD-Cu2+ solution under the assistance of a UV lamp. The as-prepared sensor can detect E. coli quantitatively with the help of a typical smartphone color-scanning application (APP). Thus, this study offers a valid dual-mode assay for sensitive and on-site visible detection of E. coli, guaranteeing the reliability of the results and is more attractive for practical use. Graphical Abstract Schematic illustration of the smartphone-integrated sensing system for fluorescent and colorimetric dual-channel detection of E. coli based on the Cu2+-OPD system.

中文翻译:

智能手机集成的纸张感应系统,用于对食源性致病细菌进行荧光和比色双通道检测。

食源性微生物引起的感染对当今的全球环境和公共医疗保健构成了巨大威胁。因此,非常需要能够实现食源性细菌鉴定的快速,便携式和灵敏的测定。在这项研究中,已经建立了一个智能的荧光和比色法双读数传感系统,该系统可通过使用Cu2 +触发的邻苯二胺(OPD)氧化来简单快速地测定大肠杆菌。最初,Cu2 +可以将OPD氧化为OPDox,从而产生橙黄色荧光和可见的浅黄色。但是,大肠杆菌可以有效地将Cu2 +还原为Cu +,从而抑制了Cu2 +触发的OPD氧化为OPDox。因此,引入大肠杆菌可以同时关闭OPD-Cu2 +系统的荧光和UV-vis吸收信号,说明了荧光和比色法双通道检测大肠杆菌的原始机制。此外,建立了基于滤纸的视觉传感器,并在紫外线灯的辅助下将其与OPD-Cu2 +溶液耦合。准备好的传感器可以借助典型的智能手机颜色扫描应用程序(APP)定量检测大肠杆菌。因此,这项研究为敏感和现场可见的大肠杆菌检测提供了有效的双模式测定法,保证了结果的可靠性,并且对实际使用更具吸引力。图形化抽象示意图,说明了基于Cu2 + -OPD系统的智能手机集成传感系统,用于大肠杆菌的荧光和比色双通道检测。建立了基于滤纸的视觉传感器,并在紫外线灯的辅助下将其与OPD-Cu2 +溶液耦合。准备好的传感器可以借助典型的智能手机颜色扫描应用程序(APP)定量检测大肠杆菌。因此,这项研究为敏感和现场可见的大肠杆菌检测提供了有效的双模式测定法,保证了结果的可靠性,并且对实际使用更具吸引力。图形化抽象示意图,说明了基于Cu2 + -OPD系统的智能手机集成传感系统,用于大肠杆菌的荧光和比色双通道检测。建立了一个基于滤纸的视觉传感器,并在紫外线灯的辅助下将其与OPD-Cu2 +溶液耦合。准备好的传感器可以借助典型的智能手机颜色扫描应用程序(APP)定量检测大肠杆菌。因此,这项研究为敏感和现场可见的大肠杆菌检测提供了有效的双模式测定法,保证了结果的可靠性,并且对实际使用更具吸引力。图形化摘要示意图,说明了基于Cu2 + -OPD系统的智能手机集成传感系统,用于大肠杆菌的荧光和比色双通道检测。这项研究为敏感和现场可见的大肠杆菌检测提供了有效的双模式测定法,保证了结果的可靠性,并且对实际使用更具吸引力。图形化摘要示意图,说明了基于Cu2 + -OPD系统的智能手机集成传感系统,用于大肠杆菌的荧光和比色双通道检测。这项研究为敏感和现场可见的大肠杆菌检测提供了有效的双模式测定法,保证了结果的可靠性,并且对实际使用更具吸引力。图形化抽象示意图,说明了基于Cu2 + -OPD系统的智能手机集成传感系统,用于大肠杆菌的荧光和比色双通道检测。
更新日期:2020-01-04
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