Biofilm Inhibitor Taurolithocholic Acid Alters Colony Morphology, Specialized Metabolism, and Virulence of Pseudomonas aeruginosa.,ACS Infectious Diseases

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Biofilm Inhibitor Taurolithocholic Acid Alters Colony Morphology, Specialized Metabolism, and Virulence of Pseudomonas aeruginosa.
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2020-01-03 , DOI: 10.1021/acsinfecdis.9b00424
Alanna R Condren ₁ , Lisa Juliane Kahl ₂ , Gabriela Boelter ₃ , George Kritikos ₃ , Manuel Banzhaf ₃ , Lars E P Dietrich ₂ , Laura M Sanchez ₁

Affiliation

Biofilm inhibition by exogenous molecules has been an attractive strategy for the development of novel therapeutics. We investigated the biofilm inhibitor taurolithocholic acid (TLCA) and its effects on the specialized metabolism, virulence, and biofilm formation of the clinically relevant bacterium Pseudomonas aeruginosa strain PA14. Our study shows that TLCA alters the specialized metabolism, thereby affecting P. aeruginosa colony biofilm physiology. We observed an upregulation of metabolites correlated to virulence such as the siderophore pyochelin. A wax moth virulence assay confirmed that treatment with TLCA increases the virulence of P. aeruginosa. On the basis of our results, we believe that future endeavors to identify biofilm inhibitors must consider how a putative lead alters the specialized metabolism of a bacterial community to prevent pathogens from entering a highly virulent state.

中文翻译：

生物膜抑制剂牛磺石胆酸会改变菌落形态，专门代谢和铜绿假单胞菌的毒性。

外源性分子对生物膜的抑制作用一直是开发新型疗法的诱人策略。我们研究了生物膜抑制剂牛磺石胆酸（TLCA）及其对临床相关细菌铜绿假单胞菌菌株PA14的专门代谢，毒力和生物膜形成的影响。我们的研究表明，TLCA会改变专门的代谢，从而影响铜绿假单胞菌菌落的生物膜生理。我们观察到与毒力相关的代谢产物上调，如铁载体泛素。蜡蛾毒力测定证实用TLCA处理可增加铜绿假单胞菌的毒力。根据我们的结果，

更新日期：2019-12-18

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