In Situ Formation of Covalent Second Network in a DNA Supramolecular Hydrogel and Its Application for 3D Cell Imaging.,ACS Applied Materials & Interfaces

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In Situ Formation of Covalent Second Network in a DNA Supramolecular Hydrogel and Its Application for 3D Cell Imaging.
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-01-14 , DOI: 10.1021/acsami.9b11534
Tianyang Cao ₁ , Haoyang Jia ₁ , Yuanchen Dong ₂ , Songbai Gui ₃ , Dongsheng Liu ₁

Affiliation

DNA hydrogels have been demonstrated with important applications in three-dimensional cell culture in vitro due to their good biocompatibility, biodegradability, and permeability. In these applications, to observe the cell morphology and functions in situ, immobilization, labeling, and imaging processes are involved, which requires good stability of the hydrogels during washing and immersion. To improve the stability of the hydrogels for better imaging, here we built a covalent second network in a DNA supramolecular hydrogel by in situ polymerization and successfully constructed a stable three-dimensional transparent system for cell culture and observation. This strategy has been proved to be efficient in enhancing the mechanical properties and immobilizing the cells inside the hydrogel, which can be applied for immunostaining and cell imaging.

中文翻译：

DNA超分子水凝胶中共价第二网络的原位形成及其在3D细胞成像中的应用。

DNA水凝胶具有良好的生物相容性，生物降解性和渗透性，已在体外三维细胞培养中得到了重要应用。在这些应用中，要原位观察细胞的形态和功能，需要进行固定，标记和成像过程，这要求水凝胶在洗涤和浸入期间具有良好的稳定性。为了提高水凝胶的稳定性，以便更好地成像，在这里我们通过原位聚合在DNA超分子水凝胶中建立了一个共价第二网络，并成功地构建了一个稳定的三维透明系统用于细胞培养和观察。事实证明，该策略在增强机械性能和固定水凝胶内部细胞方面是有效的，可用于免疫染色和细胞成像。

更新日期：2020-01-15

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