Abstract Radiation-induced skin injuries are a common side effect of radiotherapy. Plant-derived polysaccharide is considered as an attractive cosmeceutical ingredient for skin damage therapy. This study was conducted to investigate the protective effects of Annona muricata leaf polysaccharide (ALP) on radiation-induced skin injuries by using in vitro and in vivo models. In normal human epidermal keratinocytes (NHEKs), ALP treatments reduced irradiation-induced apoptosis by increasing antioxidant enzymes activities, including superoxide dismutase (SOD) and catalase. Furthermore, ALP treatments decreased levels of interleukin-1β, nucleotide-binding domain and leucine-rich-repeat-containing family pyrin 3 (NLRP3), and cleavage of caspase-1 and caspase-3. Most importantly, the topical application of the ALP cream showed protective efficacy against irradiation exposure, including the reduction of epidermal thickening, as well as an increase in the number of apoptotic cells and antioxidant enzyme (SOD and catalase) activities in skin tissue. Our results indicate that ALP can be potentially used to treat radiation-induced skin injuries.

中文翻译：

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来自番荔枝叶的多糖保护正常人表皮角质形成细胞和小鼠皮肤免受辐射损伤

摘要 放射致皮肤损伤是放射治疗的常见副作用。植物来源的多糖被认为是一种用于皮肤损伤治疗的有吸引力的药妆成分。本研究旨在通过体外和体内模型研究番荔枝叶多糖 (ALP) 对辐射诱导的皮肤损伤的保护作用。在正常人表皮角质形成细胞 (NHEK) 中，ALP 处理通过增加抗氧化酶活性，包括超氧化物歧化酶 (SOD) 和过氧化氢酶，减少辐射诱导的细胞凋亡。此外，ALP 处理降低了白细胞介素 1β、核苷酸结合域和富含亮氨酸重复序列的家族 pyrin 3 (NLRP3) 以及 caspase-1 和 caspase-3 的裂解水平。最重要的是，ALP 乳膏的局部应用显示出对辐射暴露的保护功效，包括减少表皮增厚，以及增加皮肤组织中凋亡细胞和抗氧化酶（SOD 和过氧化氢酶）的数量。我们的结果表明 ALP 可潜在用于治疗辐射引起的皮肤损伤。