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Application of the laser technology on the effect of Cd phytotoxicity in the detection of NH3, C2H4, C2H5OH and CO2 biomolecules at Triticum aestivum plantlets
Sustainable Chemistry and Pharmacy ( IF 5.5 ) Pub Date : 2020-01-03 , DOI: 10.1016/j.scp.2019.100208
Cristina Popa , Ana Maria Bratu , Mihaela Bacalum , Petronela Prepelita

Cadmium (Cd) is a nonessential heavy metal that presents a great environmental problem due to its high toxicity to all living organisms. Cadmium can obstruct plant germination, growth, and production mainly associated with the physiological, biochemical and genetic elements of the plant system. The aim of our study was to monitor the ammonia (NH3), ethylene (C2H4), ethanol (C2H5OH) and carbon dioxide (CO2) biomolecules emissions in Triticum aestivum plantlets growth with or without Cd, using photoacoustic spectroscopy in order to suggest new markers that may contribute to a better understanding of Cd phytotoxicity. At the same time, common wheat roots were imaged using fluorescence microscopy and the chemical composition analysis of the plantlet surfaces was determined using X-ray photoelectron spectroscopy. Photoacoustic spectroscopy results show that seeds germinated with Cd, determine a slowly increase of NH3 vapors in the respiration of plantlets, a decrease of C2H4, and a greater increase of C2H5OH and CO2 vapors in the respiration of Triticum aestivum. In the same time, through all techniques employed we were able also to distinguish between Triticum aestivum plantlets treated or not with Cd.



中文翻译:

激光技术在镉对植物小麦幼苗NH 3,C 2 H 4,C 2 H 5 OH和CO 2生物分子的检测中的作用

镉(Cd)是一种不必要的重金属,由于对所有生物都具有高毒性,因此会带来很大的环境问题。镉会阻碍植物的发芽,生长和生产,主要与植物系统的生理,生化和遗传因素有关。我们的研究目的是监测普通小麦中氨(NH3),乙烯(C2H4),乙醇(C2H5OH)和二氧化碳(CO2)生物分子的排放。使用光声光谱法研究有无Cd的小植株生长,以便提出可能有助于更好地了解Cd植物毒性的新标记。同时,使用荧光显微镜对普通小麦的根进行成像,并使用X射线光电子能谱确定幼苗表面的化学成分。光声光谱结果表明,种子与Cd萌发,决定了幼苗呼吸中NH 3蒸气的缓慢增加,C 2 H 4的减少,以及C 2 H 5 OH和CO 2蒸气的较大增加。普通小麦。同时,通过使用的所有技术,我们还能够区分使用或不使用镉处理过的普通小麦苗。

更新日期:2020-01-03
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