We developed a convenient technique to detect Herpesviral haematopoietic necrosis attributed to cyprinid herpes virus 2 (CyHV-2), a serious disease of Crucian carp and goldfish related to high mortality. In the present study, we employed a lateral flow dipstick (LAMP-LFD) to present a loop-mediated isothermal amplification assay. The specificity was ascertained via other six viruses, and the sensitivity was compared using PCR method, which are the reaction conditions changes for the method improved. The results revealed that CyHV-2 performance was observable at 64 °C in a separated tube within 60 min, when the samples hybridized using an FITC-labeled probe. As the LAMP-LFD method's specificity was high, with its sensitivity identical to that of traditional PCR, the overall DNA collected revealed the lowest detection limit of 0.18 pg/μl from goldfish diseased by CyHV-2. In summary, the development of LAMP-LFD's method does not require expensive instruments, and it can be regarded as a fast, simple, and reliable method for CyHV-2 detection.

中文翻译：

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通过环介导的等温扩增结合侧向量油尺检测塞浦路斯疱疹病毒2。

我们开发了一种方便的技术来检测归因于塞浦路斯疱疹病毒2（CyHV-2）的疱疹病毒性造血坏死，这是一种与高死亡率有关的鱼和金鱼的严重疾病。在本研究中，我们采用了一个侧向量油尺（LAMP-LFD）来进行回路介导的等温扩增测定。通过其他六种病毒确定特异性，并使用PCR方法比较灵敏度，这是改进方法的反应条件变化。结果显示，当样品使用FITC标记的探针杂交时，在60°C的分离管中，在64°C下可观察到CyHV-2的性能。由于LAMP-LFD方法的特异性很高，并且灵敏度与传统PCR相同，因此收集的总DNA显示最低检测限为0。来自CyHV-2感染的金鱼的18 pg /μl。总而言之，LAMP-LFD方法的开发不需要昂贵的仪器，它可以被认为是CyHV-2检测的快速，简单和可靠的方法。