In order to evaluate the genes involved in polyamines synthesis pathway and the role of nitric oxide synthase (NOS) and H2O2 in stomatal closure under drought stress, a research conducted with three irrigation levels (100, 50 and 25% field capacity) at 1, 3, 6 and 12 days on Rosa damascena Mill. HPLC and qPCR results showed that putrescine (Put) accumulation occurred at first day in both 50 and 25% of field capacity and then decreased the other days. Furthermore, Put accumulation in the indirect pathway (ADC, AIH and CPA) was more effective related to the direct pathway (ODC) under severe stress. Increased expression of genes involved in production of spermidine (Spd) and spermine (Spm) i.e., SAMDC, SPDS and SPMS correlated with the highest accumulation of Spd and Spm under 50% FC at 6 d and 25% FC at 12 d, respectively. Moreover, results showed that Put reduction simultaneously accumulated H2O2, which subsequently increased NOS expression suggesting a key signal for stomatal closure.

中文翻译：

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Rosa damascena Mill上气孔关闭中涉及多胺合成途径和一氧化氮合酶的基因和代谢物的作用。在干旱压力下。

为了评估多胺合成途径中涉及的基因以及一氧化氮合酶（NOS）和H2O2在干旱胁迫下气孔关闭中的作用，在三种灌溉水平（田间持水量分别为100％，50％和25％）下进行了一项研究， Rosa damascena Mill的第3、6和12天。HPLC和qPCR结果表明，第一天在50％和25％的田间持水量中都会发生腐胺（Put）积累，然后在其他几天减少。此外，在严重压力下，间接途径（ADC，AIH和CPA）中的Put积累与直接途径（ODC）更有效。参与产生亚精胺（Spd）和亚精胺（Spm）的基因表达的增加，即SAMDC，SPDS和SPMS与分别在6 d的50％FC和12 d的25％FC下Spd和Spm的最高积累相关。此外，