Troponin C (TnC) is one member of the EF-hand superfamily. In many species, this gene had been identified and related functions had been elucidated. The TnC gene was still blank in the Scylla paramamosain. We obtained the TnC gene for the first time in the S. paramamosain. And we systematically analyzed the possible role of this gene in the innate immunity of S. paramamosain while infected with white spot syndrome virus (WSSV) or Vibrio alginolyticus. The full-length 1427 bp sequence of TnC contains a 453 bp open reading frame (ORF) for encoding a 151 amino acid protein. Detection of tissue specificity of gene expression showed that the TnC was primarily expressed in muscle tissue. The expression of TnC was successfully inhibited by RNA interference technology, and several immune genes were affected. The activity of phenoloxidase and superoxide dismutase increased, and the total hemocytes counts increased after RNAi of TnC. It was found that after infection with V. alginolyticus and WSSV, the expression of TnC in hemocytes decreased. Infected with V. alginolyticus and WSSV, the cumulative mortality and apoptotic rate of hemocytes increased after silencing the TnC gene. Our results indicate that TnC takes participate in the innate immunity of S. paramamosain and may plays a different role in the antiviral and antibacterial immune response.

中文翻译：

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Scylla paramamosain中肌钙蛋白C（TnC）的分子表征及其在白斑综合症病毒和溶藻弧菌感染中的作用。

肌钙蛋白C（TnC）是EF手超家族的成员之一。在许多物种中，已经鉴定出该基因，并阐明了相关功能。在Sylla paramamosain中，TnC基因仍为空白。我们首次在副嗜乳链球菌中获得了TnC基因。并且我们系统地分析了该基因在感染白斑综合症病毒（WSSV）或溶藻弧菌时在副嗜酸链球菌固有免疫中的可能作用。TnC的全长1427 bp序列包含一个453 bp的开放阅读框（ORF），用于编码151个氨基酸的蛋白质。基因表达的组织特异性检测表明，TnC主要在肌肉组织中表达。RNA干扰技术成功抑制了TnC的表达，并影响了多个免疫基因。TnC RNAi后，酚氧化酶和超氧化物歧化酶的活性增加，总血细胞计数增加。发现溶藻弧菌和WSSV感染后，血细胞中TnC的表达降低。TnC基因沉默后，溶血弧菌和WSSV感染后，血细胞的累积死亡率和凋亡率增加。我们的结果表明TnC参与了S. paramamosain的先天免疫，并且可能在抗病毒和抗菌免疫应答中起不同的作用。TnC基因沉默后，血细胞的累积死亡率和凋亡率增加。我们的结果表明TnC参与了S. paramamosain的先天免疫，并且可能在抗病毒和抗菌免疫应答中起不同的作用。TnC基因沉默后，血细胞的累积死亡率和凋亡率增加。我们的结果表明TnC参与了S. paramamosain的先天免疫，并且可能在抗病毒和抗菌免疫应答中起不同的作用。