Alachlor, a chloroacetanilide endocrine disruptor herbicide is precarious for humans as well as the environment. Though banned by the European Union and classified as moderately hazardous by WHO, yet it is nevertheless used in several countries posing austere human and environmental health issues. Alachlor attenuation was scrutinized through simulated biodegradation experiments using soil-isolated microbes. Bio-disintegrative assays of pure three fungal and one bacterial strain; Aspergillus flavus (AF), Penicillium chrysogenum (PC), Aspergillus niger (AN) and Xanthomonas axonopodis (XA), respectively were utilized. Initial Alachlor concentration (10 mg/L) was prepared with individual microbial suspension and monitored for 35 d. Alachlor bio-transformation was analyzed quantitatively and qualitatively by gas chromatography mass spectroscopy. XA and AN displayed maximal potential to metabolise the herbicide while forming residues; 1-chloroacetyl, 2,3- dihydro-7 ethylindole, 7 ethylindole, 7-ethyl-3-methyl-2-methoxy-2,3-dihydroindole, N- (2,6-diethylphenyl)-methyleneamine and 7-Ethyl-N-methylindole. Alachlor degradation by AF, PC, AN and XA was found to be 17.1%, 5.5%, 72.6% and 82.1%, respectively, after 35 d. Microbes have displayed cometabolism as the main mechanism for Alachlor degradation. This research can influence imperative and significant environmental friendly bio-remedial strategies for xenobiotic eradication.

中文翻译：

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环境污染物2-氯-N-（2,6-二乙基苯基）-N-（甲氧基甲基）乙酰胺通过黄单胞菌和黑曲霉的修复。

甲草胺是一种氯乙酰苯胺内分泌干扰物除草剂，它对人类和环境均不稳定。尽管它已被欧盟禁止并被世界卫生组织列为中度危险，但是它仍然在一些提出严峻的人类和环境健康问题的国家中使用。通过使用土壤分离的微生物进行的模拟生物降解实验来详细检查甲草胺的衰减。纯的三种真菌和一种细菌菌株的生物分解测定；分别使用了黄曲霉（AF），产黄青霉（PC），黑曲霉（AN）和轴生黄单胞菌（XA）。用单独的微生物悬浮液制备甲草胺的初始浓度（10 mg / L），并监测35 d。通过气相色谱质谱法定量和定性分析了甲草胺的生物转化。XA和AN在形成残留物时具有最大的代谢除草剂的潜力。1-氯乙酰基，2,3-二氢-7乙基吲哚，7乙基吲哚，7-乙基-3-甲基-2-甲氧基-2,3-二氢吲哚，N-（2,6-二乙基苯基）-亚甲基胺和7-乙基- N-甲基吲哚。在35 d后，AF，PC，AN和XA对甲草胺的降解分别为17.1％，5.5％，72.6％和82.1％。微生物已经显示出新陈代谢是甲草胺降解的主要机制。这项研究可以影响到必须的，重要的环境友好型生物修复策略。在35 d后，AF，PC，AN和XA对甲草胺的降解分别为17.1％，5.5％，72.6％和82.1％。微生物已经显示出新陈代谢是甲草胺降解的主要机制。这项研究可以影响到必须的，重要的环境友好型生物修复策略。在35 d后，AF，PC，AN和XA对甲草胺的降解分别为17.1％，5.5％，72.6％和82.1％。微生物已显示出新陈代谢是甲草胺降解的主要机制。这项研究可以影响到必须的，重要的环境友好型生物修复策略。