Strong discrepancies in published data on the levels and epitope specificities of antibodies against the xenogenic N-glycolyl forms of sialoglycans (Hanganutziu-Deicher Neu5Gcɑ2-3Galβ1-4Glc and related antigens) in healthy donors prompted us to carry out a systematic study in this area using the printed glycan array and other methods. This article summarizes and discusses our published and previously unpublished data, as well as publicly available data from the Consortium for Functional Glycomics. As a result, we conclude that (1) the level of antibodies referred to as anti-Neu5Gc in healthy individuals is low; (2) there are antibodies that seem to interact with Neu5Gc-containing epitopes, but in fact they recognize internal fragments of Neu5Gc-containing glycans (without sialic acids), which served as antigens in the assays used and; (3) a population capable of interacting specifically with Neu5Gc (it does not bind the corresponding NAc analogs) does exist, but it binds the monosaccharide Neu5Gc better than the entire glycans containing it. In other words, in healthy donors, there are populations of antibodies capable of binding the Neu5Gc monosaccharide or the inner core -Galβ1-4Glc, but very few true anti-Neu5Gcɑ2-3Galβ1-4Glc antibodies, i.e., antibodies capable of specifically recognizing the entire trisaccharide.

中文翻译：

---

健康人的血液中是否存在针对 Neu5Gc 表位的特异性抗体？

健康供体中针对异种 N-甘醇形式唾液酸聚糖（Hanganutziu-Deicher Neu5Gcɑ2-3Galβ1-4Glc 和相关抗原）的抗体水平和表位特异性的已发表数据存在很大差异，这促使我们在该领域进行系统研究，使用打印的聚糖阵列和其他方法。本文总结并讨论了我们已发布和之前未发布的数据，以及来自功能糖组学联盟的公开数据。因此，我们得出结论：（1）健康个体中被称为抗 Neu5Gc 的抗体水平较低；(2) 有些抗体似乎与含有 Neu5Gc 的表位相互作用，但实际上它们识别含有 Neu5Gc 的聚糖（不含唾液酸）的内部片段，这些片段在所用的测定中用作抗原；(3) 确实存在能够与 Neu5Gc 特异性相互作用的群体（它不结合相应的 NAc 类似物），但它与单糖 Neu5Gc 的结合比包含它的整个聚糖更好。换言之，在健康供体中，存在能够结合 Neu5Gc 单糖或内核 -Galβ1-4Glc 的抗体群体，但很少有真正的抗 Neu5Gcɑ2-3Galβ1-4Glc 抗体，即能够特异性识别整个三糖。