DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics.,PLOS Genetics

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DNA double strand break repair in Escherichia coli perturbs cell division and chromosome dynamics.
PLOS Genetics ( IF 4.0 ) Pub Date : 2020-01-02 , DOI: 10.1371/journal.pgen.1008473
Martin A White _{1,

2} , Elise Darmon ₁ , Manuel A Lopez-Vernaza ₁ , David R F Leach ₁

Affiliation

To prevent the transmission of damaged genomic material between generations, cells require a system for accommodating DNA repair within their cell cycles. We have previously shown that Escherichia coli cells subject to a single, repairable site-specific DNA double-strand break (DSB) per DNA replication cycle reach a new average cell length, with a negligible effect on population growth rate. We show here that this new cell size distribution is caused by a DSB repair-dependent delay in completion of cell division. This delay occurs despite unperturbed cell size regulated initiation of both chromosomal DNA replication and cell division. Furthermore, despite DSB repair altering the profile of DNA replication across the genome, the time required to complete chromosomal duplication is invariant. The delay in completion of cell division is accompanied by a DSB repair-dependent delay in individualization of sister nucleoids. We suggest that DSB repair events create inter-sister connections that persist until those chromosomes are separated by a closing septum.

中文翻译：

大肠杆菌中的 DNA 双链断裂修复扰乱细胞分裂和染色体动力学。

为了防止受损基因组材料在世代之间传递，细胞需要一个系统来适应其细胞周期内的 DNA 修复。我们之前已经表明，每个 DNA 复制周期受到单个、可修复的位点特异性 DNA 双链断裂 (DSB) 的大肠杆菌细胞会达到新的平均细胞长度，对种群增长率的影响可以忽略不计。我们在这里展示了这种新的细胞大小分布是由细胞分裂完成的 DSB 修复依赖延迟引起的。尽管不受干扰的细胞大小调节了染色体 DNA 复制和细胞分裂的启动，但仍会发生这种延迟。此外，尽管 DSB 修复改变了整个基因组的 DNA 复制谱，但完成染色体复制所需的时间是不变的。细胞分裂完成的延迟伴随着姐妹核仁个体化的 DSB 修复依赖性延迟。我们建议 DSB 修复事件创建姐妹间连接，这种连接一直持续到这些染色体被关闭的隔膜分开。

更新日期：2020-02-18

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