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A phantom system for assessing the effects of membrane lipids on water proton relaxation.
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-01-03 , DOI: 10.1002/nbm.4209 Oshrat Shtangel 1 , Aviv A Mezer 1
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-01-03 , DOI: 10.1002/nbm.4209 Oshrat Shtangel 1 , Aviv A Mezer 1
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Quantitative MRI (qMRI) is a method for the non-invasive study of brain-structure-associated changes expressed with measurable units. The qMRI-derived parameters have been shown to reflect brain tissue composition such as myelin content. Nevertheless, it remains a major challenge to identify and quantify the contributions of specific molecular components to the MRI signal. Here, we describe a phantom system that can be used to evaluate the contribution of membrane lipids to qMRI-derived parameters. We used a hydration-dehydration dry film technique to formulate liposomes that can be used as a model of the bilayer lipid membrane. The liposomes were comprised of the most abundant types of lipid found in the human brain. We then applied clinically available qMRI techniques with adjusted bias corrections in order to test the ability of the phantom system to estimate multiple qMRI parameters such as proton density (PD), T1 , T2 , T2 * and magnetization transfer. In addition, we accurately measured the phantom sample water fraction (normalized PD). A similar protocol was also applied to the human brain in vivo. The phantom system allows for a reliable estimation of qMRI parameters for phantoms composed of various lipid types using a clinical MRI scanner. We also found a comparable reproducibility between the phantom and in vivo human brain qMRI estimations. To conclude, we have successfully created a biologically relevant liposome phantom system whose lipid composition can be fully controlled. Our lipid system and analysis can be used to measure the contributions to qMRI parameters of membrane lipids found in the human brain under scanning conditions that are relevant to in vivo human brain scans. Such a model system can be used to test the contributions of lipidomic changes in normal and pathological brain states.
中文翻译：
用于评估膜脂质对水质子弛豫的影响的幻影系统。
定量MRI（qMRI）是一种以可测量单位表达的与脑结构相关变化的非侵入性研究方法。qMRI衍生的参数已显示出可反映脑组织组成，例如髓磷脂含量。然而，鉴定和定量特定分子组分对MRI信号的贡献仍然是主要挑战。在这里，我们描述了一个幻影系统，可用于评估膜脂质对qMRI衍生参数的贡献。我们使用了水合脱水干膜技术来配制脂质体，该脂质体可用作双层脂质膜的模型。脂质体由在人脑中发现的最丰富的脂质组成。然后，我们应用了具有可调整的偏差校正的临床可用qMRI技术，以测试体模系统评估多个qMRI参数（如质子密度（PD），T1，T2，T2 *和磁化转移）的能力。此外，我们准确地测量了幻影样品的水含量（归一化PD）。类似的方案也被应用于体内人脑。体模系统允许使用临床MRI扫描仪可靠地估算由各种脂质类型组成的体模的qMRI参数。我们还发现了幻影和体内人脑qMRI估计值之间具有可比性。总而言之，我们已经成功创建了一个生物学相关的脂质体模型，其脂质成分可以得到完全控制。我们的脂质系统和分析可用于测量与体内人脑扫描相关的扫描条件下在人脑中发现的膜脂质对qMRI参数的贡献。这样的模型系统可以用来测试脂质组学变化在正常和病理性脑状态中的作用。
更新日期：2020-03-09
中文翻译：
用于评估膜脂质对水质子弛豫的影响的幻影系统。
定量MRI（qMRI）是一种以可测量单位表达的与脑结构相关变化的非侵入性研究方法。qMRI衍生的参数已显示出可反映脑组织组成，例如髓磷脂含量。然而，鉴定和定量特定分子组分对MRI信号的贡献仍然是主要挑战。在这里，我们描述了一个幻影系统，可用于评估膜脂质对qMRI衍生参数的贡献。我们使用了水合脱水干膜技术来配制脂质体，该脂质体可用作双层脂质膜的模型。脂质体由在人脑中发现的最丰富的脂质组成。然后，我们应用了具有可调整的偏差校正的临床可用qMRI技术，以测试体模系统评估多个qMRI参数（如质子密度（PD），T1，T2，T2 *和磁化转移）的能力。此外，我们准确地测量了幻影样品的水含量（归一化PD）。类似的方案也被应用于体内人脑。体模系统允许使用临床MRI扫描仪可靠地估算由各种脂质类型组成的体模的qMRI参数。我们还发现了幻影和体内人脑qMRI估计值之间具有可比性。总而言之，我们已经成功创建了一个生物学相关的脂质体模型，其脂质成分可以得到完全控制。我们的脂质系统和分析可用于测量与体内人脑扫描相关的扫描条件下在人脑中发现的膜脂质对qMRI参数的贡献。这样的模型系统可以用来测试脂质组学变化在正常和病理性脑状态中的作用。
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