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Neurotoxicity of β-HgS differs from environmental mercury pollutants (MeHgCl and HgCl2) in Neuro-2a cell
International Journal of Environmental Health Research ( IF 2.2 ) Pub Date : 2019-12-03 , DOI: 10.1080/09603123.2019.1692792
Zhenghua Xia 1, 2, 3 , Hongtao Bi 1, 3 , Cen Li 1, 3 , Lujing Geng 1, 2, 3 , Muhammad Usman 4 , Yuzhi Du 1, 3 , Lixin Wei 1, 3
Affiliation  

ABSTRACT

β-HgS, differing from environmental mercury pollutants (MeHgCl and HgCl2) in chemical form, is used as traditional medicine in Asian countries for thousands of years. In this study, Neuro-2a cells were exposed to β-HgS, MeHgCl and HgCl2 (5 µM) for 6–24 h. The cell viability of β-HgS was higher than MeHgCl with 25.9% and 72.4% in 12 h and 24 h respectively. As the incubation time increased, MeHgCl had obvious damage to cell morphology, decreased the ratio of Bcl-2 and Bak and increased the expressions of TNF-α, IL-6 and IL-1β significantly. Furthermore, the expressions of IL-1β and IL-6 in HgCl2 group were increased significantly in 6 h and 24 h. The apoptotic rates in MeHgCl and HgCl2 group were respectively higher than β-HgS with 32.2% and 7.30% in 24 h. Our findings indicate that β-HgS is much less neurotoxicity than MeHgCl and HgCl2 in Neuro-2a cells.



中文翻译:

β-HgS 的神经毒性与 Neuro-2a 细胞中的环境汞污染物(MeHgCl 和 HgCl2)不同

摘要

β-HgS在化学形式上与环境汞污染物(MeHgCl 和 HgCl 2)不同,在亚洲国家作为传统药物已有数千年历史。在本研究中,Neuro-2a 细胞暴露于 β-HgS、MeHgCl 和 HgCl 2 (5 µM) 6-24 小时。β-HgS 的细胞活力高于 MeHgCl,在 12 小时和 24 小时内分别为 25.9% 和 72.4%。随着孵育时间的增加,MeHgCl对细胞形态有明显的破坏,Bcl-2和Bak的比例降低,TNF-α、IL-6和IL-1β的表达显着增加。此外,HgCl 2组IL-1β和IL-6的表达在6 h和24 h显着增加。MeHgCl 和 HgCl 2 中的细胞凋亡率24 h 组分别高于 β-HgS 32.2% 和 7.30%。我们的研究结果表明,在 Neuro-2a 细胞中,β-HgS 的神经毒性比 MeHgCl 和 HgCl 2小得多。

更新日期:2019-12-03
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