ABSTRACT

β-HgS, differing from environmental mercury pollutants (MeHgCl and HgCl₂) in chemical form, is used as traditional medicine in Asian countries for thousands of years. In this study, Neuro-2a cells were exposed to β-HgS, MeHgCl and HgCl₂ (5 µM) for 6–24 h. The cell viability of β-HgS was higher than MeHgCl with 25.9% and 72.4% in 12 h and 24 h respectively. As the incubation time increased, MeHgCl had obvious damage to cell morphology, decreased the ratio of Bcl-2 and Bak and increased the expressions of TNF-α, IL-6 and IL-1β significantly. Furthermore, the expressions of IL-1β and IL-6 in HgCl₂ group were increased significantly in 6 h and 24 h. The apoptotic rates in MeHgCl and HgCl₂ group were respectively higher than β-HgS with 32.2% and 7.30% in 24 h. Our findings indicate that β-HgS is much less neurotoxicity than MeHgCl and HgCl₂ in Neuro-2a cells.

摘要

β-HgS在化学形式上与环境汞污染物（MeHgCl 和 HgCl ₂）不同，在亚洲国家作为传统药物已有数千年历史。在本研究中，Neuro-2a 细胞暴露于 β-HgS、MeHgCl 和 HgCl ₂ (5 µM) 6-24 小时。β-HgS 的细胞活力高于 MeHgCl，在 12 小时和 24 小时内分别为 25.9% 和 72.4%。随着孵育时间的增加，MeHgCl对细胞形态有明显的破坏，Bcl-2和Bak的比例降低，TNF-α、IL-6和IL-1β的表达显着增加。此外，HgCl ₂组IL-1β和IL-6的表达在6 h和24 h显着增加。MeHgCl 和 HgCl _{2 中}的细胞凋亡率24 h 组分别高于 β-HgS 32.2% 和 7.30%。我们的研究结果表明，在 Neuro-2a 细胞中，β-HgS 的神经毒性比 MeHgCl 和 HgCl ₂小得多。