The highly conserved heat shock protein 70 (HSP70) contributes to survival at a cellular level and greatly enhances stress tolerance in many organisms. In this study, we isolate and characterize Cshsp702, which encodes an inducible form of HSP70 in the rice stem borer, Chilo suppressalis. Cshsp702 does not contain introns; the translational product is comprised of 629 amino acids with an isoelectric point of 5.69. Real-time quantitative PCR revealed that Cshsp702 was expressed at maximal levels in hemocytes and was minimally expressed in the midgut. Expression of Cshsp702 in response to a range of temperatures (−11 to 43 °C) indicated significant induction by extreme cold and hot temperatures, with maximum expression after 2 h at 42 °C. The induction of Cshsp702 in response to the endoparasite Cotesia chilonis was also studied; interestingly, Cshsp702 expression in C. suppressalis was significantly induced at 24 h and 5 days, which correspond to predicted times of C. chilonis feeding and growth, respectively. The potential induction of Cshsp702 as an inflammatory response due to parasitic stress is discussed. In conclusion, Cshsp702 is induced in response to both environmental and biotic stress and plays an important role in the physiological adaptation of C. suppressalis.

中文翻译：

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抑制Chilo suppress中诱导型HSP70基因的表达以及响应环境和生物胁迫的表达。

高度保守的热休克蛋白70（HSP70）有助于细胞水平的存活，并大大提高了许多生物体的抗逆性。在这项研究中，我们分离和鉴定Cshsp702，其编码HSP70的诱导形式在水稻二化螟，二化螟。Cshsp702不包含内含子；翻译产物包括629个氨基酸，等电点为5.69。实时定量PCR显示，Cshsp702在血细胞中的表达最高，而在中肠中的表达则最低。Cshsp702的表达在一定温度范围内（-11至43°C）的响应表明极端的寒冷和炎热温度会产生明显的诱导作用，在42°C下2 h后表达最大。的诱导Cshsp702响应于所述体内寄生虫绒茧chilonis也进行了研究; 有趣的是，在24小时和5天显着诱导了C.抑制菌中Cshsp702的表达，这分别对应于预计的C. chilonis进食和生长时间。讨论了Cshsp702潜在诱导的由于寄生性应激引起的炎症反应。总之，Cshsp702响应于环境和生物胁迫诱导和起着的生理适应重要作用二化螟。