Alveolar and bronchial epithelial cells have critical functions in acute respiratory distress syndrome progress. Genistein could protect the lungs from acute lung injury, however, whether genistein protects the alveolar epithelial cells from LPS-induced injury was less studied. Spectrophotometric method 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and enzyme-linked immunosorbent assay (ELISA) were performed to detect cell viability and levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6. Flow cytometry and western blot assay were performed to detect cells apoptosis and protein levels. In LPS-induced model of mouse lung epithelial (MLE)-12 cells, PBEF (proinflammatory cytokine) expression, and cell apoptosis were increased and cell viability was decreased, whereas NF-κB was activated and expression levels of TNF-α, IL-1β, and IL-6 were increased. However, genistein partly reversed the effect of LPS, and it plays a protective role in lung injury by reducing expression of PBEF, inhibiting the activation of NF-κB and alleviating inflammatory response of cells.

中文翻译：

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金雀异黄素对脂多糖诱导的小鼠肺泡上皮细胞损伤的作用及其机制。

肺泡和支气管上皮细胞在急性呼吸窘迫综合征的进展中具有关键功能。金雀异黄素可以保护肺免受急性肺损伤的侵害，但是，金雀异黄素是否可以保护肺泡上皮细胞免受LPS诱导的损伤的研究较少。进行了分光光度法3- [4,5-二甲基噻唑-2-基] -2,5-二苯基四唑鎓（MTT）和酶联免疫吸附测定（ELISA）的检测细胞活力和肿瘤坏死因子-α（TNF）的水平-α），白介素-1β（IL-1β）和IL-6。进行流式细胞术和蛋白质印迹试验以检测细胞凋亡和蛋白质水平。在LPS诱导的小鼠肺上皮（MLE）-12细胞模型中，PBEF（促炎性细胞因子）表达和细胞凋亡增加，细胞活力降低，NF-κB被激活，TNF-α，IL-1β和IL-6的表达水平升高。然而，金雀异黄素部分地逆转了LPS的作用，并且通过减少PBEF的表达，抑制NF-κB的活化和减轻细胞的炎症反应而在肺损伤中起保护作用。