Understanding the molecular mechanisms of endogenous and environmental metabolites is crucial for basic biology and drug discovery. With the genome, proteome, and metabolome of many organisms being readily available, researchers now have the opportunity to dissect how key metabolites regulate complex cellular pathways in vivo. Nonetheless, characterizing the specific and functional protein targets of key metabolites associated with specific cellular phenotypes remains a major challenge. Innovations in chemical biology are now poised to address this fundamental limitation in physiology and disease. In this review, we highlight recent advances in chemoproteomics for targeted and proteome-wide analysis of metabolite-protein interactions that have enabled the discovery of unpredicted metabolite-protein interactions and facilitated the development of new small molecule therapeutics.

中文翻译：

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代谢物-蛋白质相互作用的靶向和蛋白质组范围分析。

了解内源性和环境代谢物的分子机制对于基础生物学和药物发现至关重要。随着许多生物体的基因组、蛋白质组和代谢组随时可用，研究人员现在有机会剖析关键代谢物如何在体内调节复杂的细胞通路。尽管如此，表征与特定细胞表型相关的关键代谢物的特异性和功能性蛋白质靶标仍然是一项重大挑战。化学生物学的创新现在准备解决生理学和疾病的这一基本限制。在这篇评论中，