Abstract

Chronic exposure to solar ultraviolet (UV) light induces photoaging in human skin. Our previous results have shown that areca nut procyanidins (ANPs) have antioxidant capacity and possess potential anti-inflammatory effects. Here, we aimed to investigate the effect of ANPs on UVB-induced photoaging. In the present study, dorsal skin of CD-1 mice was exposed to UVB at a minimal erythema dose (130 mJ/cm²) throughout a 3-week period. The effects of ANPs and epigallocatechin-3-gallate (EGCG), a polyphenolic constituent of green tea, on UVB-induced photoaging were compared. The results show that oral administration of ANP prevented UVB-induced photoaging, indicated by epidermal thickness and collagen disorientation, and inhibited UVB-induced expression of cyclooxygenase-2 and matrix metalloproteinases (MMPs), such as MMP-2, MMP-9, and TIMP1. The protective potential of ANP on UVB-induced photodamage was comparable to that of EGCG. These data suggest that ANP could be useful as a dietary supplement to attenuate solar UVB-induced premature skin aging.

摘要

长期暴露于太阳紫外线 (UV) 会导致人体皮肤光老化。我们之前的研究结果表明，槟榔原花青素 (ANPs) 具有抗氧化能力并具有潜在的抗炎作用。在这里，我们旨在研究 ANPs 对 UVB 诱导的光老化的影响。在本研究中，CD-1 小鼠的背部皮肤以最小红斑剂量 (130 mJ/cm ²) 在整个 3 周期间。比较了 ANP 和绿茶的多酚成分表没食子儿茶素 3-没食子酸酯 (EGCG) 对 UVB 诱导的光老化的影响。结果表明，口服 ANP 可防止 UVB 诱导的光老化，表现为表皮厚度和胶原蛋白定向障碍，并抑制 UVB 诱导的环氧合酶 2 和基质金属蛋白酶 (MMP) 的表达，如 MMP-2、MMP-9 和TIMP1。ANP 对 UVB 引起的光损伤的保护潜力与 EGCG 相当。这些数据表明，ANP 可用作膳食补充剂，以减轻太阳 UVB 引起的皮肤过早老化。