Introduction: As it is generally known, regulatory B cells (Bregs) control inflammation and autoimmunity. The significance of Bregs in the population of children with autoimmune thyroid diseases (AITD) still offers plenty of potential to explore. The aim of this study was to estimate the expression of Bregs (phenotype CD19+CD24+CD27+IL-10+, CD19+IL-10+, CD1d+CD5+CD19+IL-10+ and CD1d+CD5+CD19+CD24+CD27+) in a paediatric cohort with AITD and in health controls.Materials and methods: A total of 100 blood samples were obtained from 53 paediatric patients with Graves' disease (GD) (N = 12 newly diagnosed, mean age 12.5 ± 3.5 and N = 17 during methimazole therapy, mean age 12.7 ± 4.4), Hashimoto's thyroiditis (HT) (N = 10 newly diagnosed, mean age 13.3 ± 2.9 and N = 10 during L-thyroxine therapy, mean age 13.7 ± 3.4) and compared with healthy controls (C) (N = 15, mean age 13.1 ± 3.1). The expressions of the immune cell populations were analysed by four-color flow cytometry using a FASC Canto II cytometer (BD Biosciences).Results: There was a decreasing tendency in the number of lymphocytes B producing IL-10 (B10) cells among all B lymphocytes and more widely, also among all lymphocytes, in each study group, as compared to C. We reported a reduction in IL-10 production in Bregs with the expression of CD19+CD24+CD27+IL-10 and CD1d+CD5+CD19+IL-10+ in both untreated and treated AITD.Conclusions: Our data demonstrate that the reduction in the number of Bregs with CD19+CD24+CD27+IL-10+ and CD19+IL-10+ expression could be responsible for breaking immune tolerance and for AITD development in children.

中文翻译：

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在患有自身免疫性甲状腺疾病的儿童中，CD19 + IL-10 +和CD19 + CD24 + CD27 +的比例较低，但CD1d + CD5 + CD19 + CD24 + CD27 + IL-10 + B细胞的比例较低。

简介：众所周知，调节性B细胞（Bregs）控制炎症和自身免疫。Bregs在患有自身免疫性甲状腺疾病（AITD）的儿童人群中的意义仍然提供了巨大的探索潜力。这项研究的目的是评估Bregs（表型CD19 + CD24 + CD27 + IL-10 +，CD19 + IL-10 +，CD1d + CD5 + CD19 + IL-10 +和CD1d + CD5 + CD19 + CD24的表达）材料和方法：从53名患有Graves病（GD）的小儿患者（N =新诊断的12名，平均年龄12.5±3.5岁，以及甲巯咪唑治疗期间N = 17，平均年龄12.7±4.4），桥本甲状腺炎（HT）（N = 10新诊断，平均年龄13.3±2.9，L =甲状腺素治疗期间N = 10，平均年龄13.7±3。4），并与健康对照组（C）进行比较（N = 15，平均年龄13.1±3.1）。用FASC Canto II细胞计数器（BD Biosciences）通过四色流式细胞术分析免疫细胞群的表达。结果：所有B细胞中产生IL-10（B10）的淋巴细胞B数量均呈下降趋势。与C相比，每个研究组中的淋巴细胞分布更为广泛，在所有淋巴细胞中也是如此。我们报道了Bregs中IL-10生成的减少，其表达为CD19 + CD24 + CD27 + IL-10和CD1d + CD5 + CD19结论：我们的数据表明，CD19 + CD24 + CD27 + IL-10 +和CD19 + IL-10 +表达导致的Breg数量减少可能是导致免疫系统破坏的原因。耐受力和儿童AITD的发展。