3' Untranslated regions (3' UTRs) of mRNAs emerged as central regulators of cellular function because they contain important but poorly characterized cis-regulatory elements targeted by a multitude of regulatory factors. The model nematode Caenorhabditis elegans is ideal to study these interactions because it possesses a well-defined 3' UTRome. To improve its annotation, we have used a genome-wide bioinformatics approach to download raw transcriptome data for 1088 transcriptome data sets corresponding to the entire collection of C. elegans trancriptomes from 2015 to 2018 from the Sequence Read Archive at the NCBI. We then extracted and mapped high-quality 3'-UTR data at ultradeep coverage. Here, we describe and release to the community the updated version of the worm 3' UTRome, which we named 3' UTRome v2. This resource contains high-quality 3'-UTR data mapped at single-base ultraresolution for 23,084 3'-UTR isoform variants corresponding to 14,788 protein-coding genes and is updated to the latest release of WormBase. We used this data set to study and probe principles of mRNA cleavage and polyadenylation in C. elegans The worm 3' UTRome v2 represents the most comprehensive and high-resolution 3'-UTR data set available in C. elegans and provides a novel resource to investigate the mRNA cleavage and polyadenylation reaction, 3'-UTR biology, and miRNA targeting in a living organism.

中文翻译：

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秀丽隐杆线虫3'UTRome v2资源用于研究mRNA切割和聚腺苷酸化，3'-UTR生物学以及miRNA靶向。

mRNA的3'非翻译区（3'UTR）成为细胞功能的中央调节因子，因为它们包含重要的，但表征较差的顺式调控元件，这些元件被多种调控因子所靶向。模型线虫秀丽隐杆线虫是研究这些相互作用的理想选择，因为它具有定义明确的3'UTRome。为了改善其注释，我们使用了全基因组生物信息学方法从NCBI的Sequence Read Archive中下载了1088个转录组数据集的原始转录组数据，这些数据集与2015年至2018年线虫转录组的整个集合相对应。然后，我们以超深覆盖率提取并映射了高质量3'-UTR数据。在这里，我们描述并向社区发布了蠕虫3'UTRome的更新版本，我们将其命名为3'UTRome v2。此资源包含高质量的3'-UTR数据，以对应于14,788个蛋白质编码基因的23,084个3'-UTR亚型变异体以单碱基超分辨率进行了映射，并已更新至最新版本的WormBase。我们使用此数据集来研究和探查秀丽隐杆线虫中的mRNA切割和聚腺苷酸化原理。蠕虫3'UTRome v2代表秀丽隐杆线虫中最全面，最高分辨率的3'-UTR数据集，并提供了新颖的资源研究了在活生物体中的mRNA切割和聚腺苷酸化反应，3'-UTR生物学以及miRNA靶向。