Thimerosal (THIM) is a common preservative used in many pharmaceutical drugs, vaccines, cosmetics and many other products. Today, it was somewhat clear that Thimerosal (THIM) is a neurotoxicant preservative. We aimed to use of a suitable agent for preventing of THIM side effects on brain. Therefore, in this research, the protective effects of Alpha Lipoic Acid (ALA), against THIM-induced brain cell loss, changes in neuroimmune cell and enzymatically contents were examined. Male Wistar rats (n = 60) were randomly distributed into five groups: 1- THIM group; this group received THIM at dose of 300 μg /kg on 7, 9, 11, 15 days after birth 2- ALA group; received ALA (20 mg/kg) in the same order. 3- THIM & ALA group; this group received ALA in the same dose, 30 minute before THIM administration.4& 5; Saline and ALA vehicle groups were also included. At 56th postnatal day, samples of the prefrontal cortex were collected and prepared for stereological, immune-histochemical, and enzymatic evaluations. The result showed that ALA, prevents the adverse effects of THIM on brain cell loss, abnormal changes in neuroimmune cells (p < 0.05), prefrontal cortex volume (p < 0.05), and the glutathione content of prefrontal cortex (p < 0.05). In conclusion, neonatal exposure to THIM can induce abnormal alterations in neuroimmune cells and brain cell density as well as prefrontal cortex volume & glutathione content, and ALA can ameliorate these abnormalities.

中文翻译：

---

硫辛酸改善 THIM 诱导的前额叶细胞丢失和发育中大鼠的异常酶含量

硫柳汞 (THIM) 是一种常见的防腐剂，用于许多药物、疫苗、化妆品和许多其他产品。今天，硫柳汞 (THIM) 是一种神经毒性防腐剂，这一点已经有些清楚了。我们旨在使用合适的药剂来预防 THIM 对大脑的副作用。因此，在本研究中，研究了硫辛酸 (ALA) 对 THIM 诱导的脑细胞损失、神经免疫细胞和酶含量变化的保护作用。雄性 Wistar 大鼠（n = 60）随机分为五组：1-THIM 组；本组在出生后第7、9、11、15天以300μg/kg的剂量接受THIM 2-ALA组；以相同的顺序接受 ALA (20 mg/kg)。3- THIM & ALA 组；该组在THIM给药前30分钟接受相同剂量的ALA。4&5；还包括盐水和ALA媒介物组。在出生后第 56 天，收集前额叶皮层样本并准备用于体视学、免疫组织化学和酶学评估。结果表明，ALA 可防止 THIM 对脑细胞丢失、神经免疫细胞异常变化 (p < 0.05)、前额叶皮层体积 (p < 0.05) 和前额叶皮层谷胱甘肽含量 (p < 0.05) 的不利影响。总之，新生儿暴露于 THIM 可引起神经免疫细胞和脑细胞密度以及前额叶皮层体积和谷胱甘肽含量的异常改变，而 ALA 可以改善这些异常。防止 THIM 对脑细胞损失、神经免疫细胞异常变化 (p < 0.05)、前额叶皮层体积 (p < 0.05) 和前额叶皮层谷胱甘肽含量 (p < 0.05) 的不利影响。总之，新生儿暴露于 THIM 可引起神经免疫细胞和脑细胞密度以及前额叶皮层体积和谷胱甘肽含量的异常改变，而 ALA 可以改善这些异常。防止 THIM 对脑细胞损失、神经免疫细胞异常变化 (p < 0.05)、前额叶皮层体积 (p < 0.05) 和前额叶皮层谷胱甘肽含量 (p < 0.05) 的不利影响。总之，新生儿暴露于 THIM 可引起神经免疫细胞和脑细胞密度以及前额叶皮层体积和谷胱甘肽含量的异常改变，而 ALA 可以改善这些异常。