Hypoglycemia-associated autonomic failure (HAAF) is a serious complication of diabetes which is associated with the absence of physiological homeostatic counter-regulatory mechanisms that are controlled by the hypothalamus and sympathetic nervous system. Identification of biomarkers for early detection of HAAF requires an advanced understanding of molecular signature of hypoglycemia which is yet to be identified. The outcomes of the present study have shown that the viability and the apoptotic rate of the hypothalamic neurons (mHypoE-N39) were decreased significantly due to hypoglycemia in a dose-dependent fashion (p < 0.05). Although there are more than 1000 miRNAs differentially expressed in hypothalamus, only twelve miRNAs (miR-7a, miR-7b, miR-9, miR-29b, miR-29c, miR-30a, miR-30b, miR-30c, miR-101b-3p, miR-181a-5p, miR-378-3p and miR-873-5p) were correlated to two main hypothalamic regulatory proteins, FOS and FTO. Expression of these proteins was very sensitive to hypoglycemia. We demonstrated that hypoglycemia modulates the expression of hypothalamic miRNAs that are related to FOS and FTO.

中文翻译：

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下丘脑 microRNA 在低血糖反应中调节 FOS 和 FTO 表达的潜在作用。


低血糖相关自主神经衰竭（HAAF）是糖尿病的严重并发症，与下丘脑和交感神经系统控制的生理稳态反调节机制的缺乏有关。鉴定用于早期检测 HAAF 的生物标志物需要对尚未确定的低血糖分子特征有深入的了解。本研究的结果表明，下丘脑神经元（mHypoE-N39）的活力和凋亡率因低血糖而显着降低，呈剂量依赖性（p < 0.05）。尽管下丘脑中有超过1000种差异表达的miRNA，但只有12种miRNA（miR-7a、miR-7b、miR-9、miR-29b、miR-29c、miR-30a、miR-30b、miR-30c、miR- 101b-3p、miR-181a-5p、miR-378-3p 和 miR-873-5p）与两种主要的下丘脑调节蛋白 FOS 和 FTO 相关。这些蛋白质的表达对低血糖非常敏感。我们证明低血糖调节与 FOS 和 FTO 相关的下丘脑 miRNA 的表达。