Hypoglycemia-associated autonomic failure (HAAF) is a serious complication of diabetes which is associated with the absence of physiological homeostatic counter-regulatory mechanisms that are controlled by the hypothalamus and sympathetic nervous system. Identification of biomarkers for early detection of HAAF requires an advanced understanding of molecular signature of hypoglycemia which is yet to be identified. The outcomes of the present study have shown that the viability and the apoptotic rate of the hypothalamic neurons (mHypoE-N39) were decreased significantly due to hypoglycemia in a dose-dependent fashion (p < 0.05). Although there are more than 1000 miRNAs differentially expressed in hypothalamus, only twelve miRNAs (miR-7a, miR-7b, miR-9, miR-29b, miR-29c, miR-30a, miR-30b, miR-30c, miR-101b-3p, miR-181a-5p, miR-378-3p and miR-873-5p) were correlated to two main hypothalamic regulatory proteins, FOS and FTO. Expression of these proteins was very sensitive to hypoglycemia. We demonstrated that hypoglycemia modulates the expression of hypothalamic miRNAs that are related to FOS and FTO.

中文翻译：

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下丘脑microRNA在响应低血糖时调节FOS和FTO表达的潜在作用。

低血糖相关的自主神经功能衰竭（HAAF）是糖尿病的严重并发症，与缺乏下丘脑和交感神经系统控制的生理性体内平衡反调节机制有关。鉴定用于早期检测HAAF的生物标记物需要对尚未确定的低血糖的分子标记有深入的了解。本研究的结果表明，由于血糖过低，下丘脑神经元（mHypoE-N39）的活力和凋亡率以剂量依赖的方式显着降低（p <0.05）。尽管下丘脑中有1000多种miRNA差异表达，但只有十二种miRNA（miR-7a，miR-7b，miR-9，miR-29b，miR-29c，miR-30a，miR-30b，miR-30c，miR- 101b-3p，miR-181a-5p，miR-378-3p和miR-873-5p与两个主要的下丘脑调节蛋白FOS和FTO相关。这些蛋白的表达对低血糖非常敏感。我们证明低血糖症调节与FOS和FTO相关的下丘脑miRNA的表达。