BACKGROUND Surgical treatments such as guided tissue regeneration (GTR) and access flap surgery are widely employed for the treatment of intrabony defects. However, little is known regarding the postoperative expression of gingival crevicular fluid (GCF) markers. OBJECTIVE The aim of this systematic review was to compare the expression of GCF markers following treatment of periodontal intrabony defects with guided tissue regeneration or access surgery. The association of the markers' expression with the clinical outcome was also assessed. METHODS An electronic literature search was conducted in MEDLINE, EMBASE, OpenGrey, LILACS and Cochrane Library up to December 2018 complemented by a manual search. Human, prospective clinical studies were identified. The changes from baseline up to 30 days (early healing) and 3 months (late healing) were assessed. RESULTS A total of 164 publications were identified and reviewed for eligibility. Of these, 10 publications fulfilled the inclusion criteria. The included studies evaluated 15 different GCF markers with a follow-up time between 21 and 360 days postoperatively. PDGF, VEGF and TIMP-1 changes were often investigated in the included studies; however, contrasting results were reported. Two studies agreed that both GTR and OFD lead to similar OPG level changes. TGF-β1 is increased early postoperatively, irrespective of the surgical technique employed. CONCLUSION There is limited evidence available on the expression of GCF markers after surgical interventions of intrabony periodontal defects. However, OPG and TGF-β1 tend to increase early post-operatively, irrespective of the surgical technique employed, irrespective of the surgical technique employed. CLINICAL RELEVANCE More well-designed, powered studies with sampling periods reflecting the regenerative process are needed, and future research should focus on employing standardised protocols for collecting, storing and analysing GCF markers.

中文翻译：

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外科治疗后骨内缺损的早期和晚期愈合期间牙龈沟液标志物的表达：系统评价。

背景技术诸如引导组织再生（GTR）和接近皮瓣手术的外科治疗被广泛地用于骨内缺损的治疗。然而，关于牙龈沟液（GCF）标记物的术后表达知之甚少。目的本系统综述的目的是比较在牙周骨内缺损与引导下的组织再生或介入手术治疗后GCF标志物的表达。还评估了标志物表达与临床结果的关联。方法截至2018年12月，在MEDLINE，EMBASE，OpenGrey，LILACS和Cochrane Library中进行了电子文献检索，并辅以人工检索。确定了人类前瞻性临床研究。评估了从基线到30天（早期康复）和3个月（晚期康复）的变化。结果总共确定了164种出版物并进行了资格审查。其中，有10家出版物符合纳入标准。纳入的研究评估了15种不同的GCF标志物，术后随访时间为21至360天。PDGF，VEGF和TIMP-1的变化通常在纳入研究中进行研究。但是，报告了相反的结果。两项研究一致认为，GTR和OFD都会导致类似的OPG水平变化。不管采用何种手术技术，术后早期TGF-β1都会升高。结论骨内牙周缺损的外科手术后，有关GCF标志物表达的证据有限。然而，OPG和TGF-β1倾向于在术后早期增加，与所使用的手术技术无关，与所使用的手术技术无关。临床相关性需要更精心设计的，功能强大的研究，其采样周期反映再生过程，并且未来的研究应集中于采用标准化协议收集，存储和分析GCF标记。