Carbon dioxide (CO₂) exposure is a common method of anesthesia in studies of Drosophila melanogaster. A number of negative side effects of CO₂ anesthesia have been reported. It is not clear whether the length of CO₂ anesthesia time affects Drosophila survival in aging research. Here, we examined the potential effect of the CO₂ anesthesia time length of 10–150 min. We found that long CO₂ exposure could lead to Drosophila death, more significant in males. The longer the anesthesia time is, the longer it takes for flies to wake up. Long‐time CO₂ anesthesia can reduce the lifespan. Our stress tests showed that long‐time CO₂ anesthesia can increase the average survival time in both males and females under starvation conditions, but can only increase female lifespan under H₂O₂ oxidative stress. Long‐time CO₂ anesthesia also significantly affects physiological traits, with spontaneous activity increased in females but decreased in males, and reduced female fecundity. Our study suggests that limiting the CO₂ anesthesia time and giving enough recovery time before performing physiological tests are important in Drosophila aging research.

中文翻译：

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CO2麻醉对果蝇衰老存活的研究。

二氧化碳（CO ₂）暴露是黑果蝇研究中的一种常用麻醉方法。已经报道了CO ₂麻醉的许多副作用。尚不清楚在老化研究中，CO ₂麻醉时间的长短是否会影响果蝇的存活。在这里，我们检查了CO ₂麻醉时间10–150分钟的潜在影响。我们发现长时间的CO ₂暴露可能导致果蝇死亡，在男性中更为明显。麻醉时间越长，苍蝇醒来所需的时间就越长。长期CO ₂麻醉会缩短寿命。我们的压力测试表明，长期处于饥饿状态的CO ₂麻醉可以增加男性和女性的平均存活时间，但只能增加H ₂ O ₂氧化应激下的女性寿命。长期CO ₂麻醉也会显着影响生理特征，雌性自发活动增加，而雄性活动减少，雌性生殖力降低。我们的研究表明，限制CO ₂麻醉时间并在进行生理测试之前给予足够的恢复时间对果蝇衰老研究很重要。