Anticarsia gemmatalis represents a relevant factor for lowering soybean and other legume crop productivities. Protease inhibitors affect protein degradation and reduce the availability of amino acids, impairing the development and survival of insect pests. To evaluate the possible use of proteinaceous protease inhibitors in the management of this pest, the activities of midgut proteases and the growth and development of A. gemmatalis larvae exposed to soybean Bowman–Birk trypsin‐chymotrypsin inhibitor (SBBI) and soybean Kunitz trypsin inhibitor (SKTI) were determined. The survival curves obtained using Kaplan–Meier estimators indicated that SKTI and SBBI stimulated larval survival. However, the development of A. gemmatalis was delayed, and prepupal weight decreased in the presence of both inhibitors. The results showed that SKTI and SBBI inhibited the trypsin‐like and total proteolytic activities of larvae on the 12th day after eclosion. On the 15th day after eclosion, larvae exposed to SKTI increased the activities of trypsin and total proteases. Although SKTI and SBBI did not affect the survival of the insect, they had effects on midgut proteases in a stage wherein A. gemmatalis fed voraciously, increased the larval cycle, and decreased prepupal weight. These findings provide baseline information about the potential of proteinaceous protease inhibitors to manage the velvetbean caterpillar, avoiding chemical pesticides.

中文翻译：

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暴露于不同大豆蛋白酶抑制剂的 Anticarsia gemmatalis Hübner（鳞翅目：夜蛾科）的幼虫发育和蛋白水解活性。


Anticarsia gemmatalis是降低大豆和其他豆类作物生产力的相关因素。蛋白酶抑制剂影响蛋白质降解并减少氨基酸的利用率，损害害虫的发育和生存。为了评估蛋白质蛋白酶抑制剂在该害虫管理中的可能用途、中肠蛋白酶的活性以及暴露于大豆 Bowman-Birk 胰蛋白酶-胰凝乳蛋白酶抑制剂 (SBBI) 和大豆 Kunitz 胰蛋白酶抑制剂 (SBBI) 的A. gemmatalis幼虫的生长和发育。 SKTI) 已确定。使用 Kaplan-Meier 估计器获得的存活曲线表明 SKTI 和 SBBI 刺激幼虫存活。然而，在两种抑制剂存在下， A. gemmatalis的发育被延迟，预蛹重量下降。结果表明，SKTI和SBBI在羽化后第12天抑制幼虫的胰蛋白酶样活性和总蛋白水解活性。羽化后第15天，暴露于SKTI的幼虫胰蛋白酶和总蛋白酶的活性增加。虽然 SKTI 和 SBBI 不影响昆虫的存活，但它们在A. gemmatalis贪婪取食的阶段对中肠蛋白酶产生影响，增加了幼虫周期，并降低了预蛹重量。这些发现提供了有关蛋白质蛋白酶抑制剂控制豆毛毛虫、避免使用化学农药的潜力的基线信息。