Anticarsia gemmatalis represents a relevant factor for lowering soybean and other legume crop productivities. Protease inhibitors affect protein degradation and reduce the availability of amino acids, impairing the development and survival of insect pests. To evaluate the possible use of proteinaceous protease inhibitors in the management of this pest, the activities of midgut proteases and the growth and development of A. gemmatalis larvae exposed to soybean Bowman–Birk trypsin‐chymotrypsin inhibitor (SBBI) and soybean Kunitz trypsin inhibitor (SKTI) were determined. The survival curves obtained using Kaplan–Meier estimators indicated that SKTI and SBBI stimulated larval survival. However, the development of A. gemmatalis was delayed, and prepupal weight decreased in the presence of both inhibitors. The results showed that SKTI and SBBI inhibited the trypsin‐like and total proteolytic activities of larvae on the 12th day after eclosion. On the 15th day after eclosion, larvae exposed to SKTI increased the activities of trypsin and total proteases. Although SKTI and SBBI did not affect the survival of the insect, they had effects on midgut proteases in a stage wherein A. gemmatalis fed voraciously, increased the larval cycle, and decreased prepupal weight. These findings provide baseline information about the potential of proteinaceous protease inhibitors to manage the velvetbean caterpillar, avoiding chemical pesticides.

中文翻译：

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暴露于不同大豆蛋白酶抑制剂下的双生抗CarcarmatalisHübner（鳞翅目：夜蛾科）的幼虫发育和蛋白水解活性。

产地抗龋齿是降低大豆和其他豆类作物生产力的重要因素。蛋白酶抑制剂会影响蛋白质降解并降低氨基酸的利用率，从而损害害虫的生长和存活。为了评估蛋白质蛋白酶抑制剂在控制这种害虫中的作用，中肠蛋白酶的活性以及暴露于大豆Bowman-Birk胰蛋白酶-胰凝乳蛋白酶抑制剂（SBBI）和大豆Kunitz胰蛋白酶抑制剂的双歧杆菌幼虫的生长和发育（确定）。使用Kaplan-Meier估计器获得的生存曲线表明SKTI和SBBI刺激了幼虫的生存。然而，双歧杆菌的发展 两种抑制剂的存在均会延迟和减少pu前的重量。结果表明，SKTI和SBBI在抽穗后第12天抑制了幼虫的胰蛋白酶样和总蛋白水解活性。灭苗后第15天，暴露于SKTI的幼虫增加了胰蛋白酶和总蛋白酶的活性。尽管SKTI和SBBI不会影响昆虫的存活，但它们在中足双歧杆菌进食，增加幼虫周期和降低pu前体重的阶段对中肠蛋白酶 有影响。这些发现提供了有关蛋白质蛋白酶抑制剂控制绒毛虫，避免使用化学农药的潜力的基线信息。