Abstract

The results of assembling the light-harvesting complexes in the cells of the purple sulfur bacterium Thiorhodospira (T.) sibirica strain Kir-3 while suppressing the biosynthesis of carotenoids with diphenylamine (DPA) were studied. LH2 complexes (B800-850 and B800-830) with different carotenoid composition were isolated from the cells obtained. Maximum inhibition of carotenoid biosynthesis (~90% of the control) was reached at an inhibitor concentration of 53.25 μM (9 mg/L). It was established that changes in the qualitative and quantitative composition of carotenoids do not affect the assembly of B800-830 and B800-850 complexes. It is assumed that, in the population of DPA-LH2 complexes from T. sibirica strain Kir-3, both the carotenoidless complexes and the complexes containing one or two carotenoid molecules can be assembled. These results support the hypothesis that carotenoids are not required for assembling B800-850 and B800-830 complexes.

中文翻译：

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LH2配合物（B800-850和B800-830）组装在无类胡萝卜素的硫细菌拟南芥Thiorhodospira sibirica菌株Kir-3的细胞中。

摘要

研究了在紫色硫细菌西伯利亚螺旋藻（Thiorhodospira（T.）sibirica菌株Kir-3 ）的细胞中组装集光复合物的结果，同时用二苯胺（DPA）抑制了类胡萝卜素的生物合成。从获得的细胞中分离出具有不同类胡萝卜素组成的LH2复合物（B800-850和B800-830）。在抑制剂浓度为53.25μM（9 mg / L）时，类胡萝卜素生物合成的最大抑制作用达到了对照的约90％。已经确定，类胡萝卜素的定性和定量组成的变化不会影响B800-830和B800-850复合物的组装。据推测，在来自西伯利亚烟草的DPA-LH2复合体的种群中菌株Kir-3可以组装无类胡萝卜素的复合物和含有一个或两个类胡萝卜素分子的复合物。这些结果支持这样的假说，即组装B800-850和B800-830复合物不需要类胡萝卜素。